北欧国家生产的蜂蜜中A型、B型、E型和F型肉毒杆菌的流行情况及多样性。
Prevalence and diversity of Clostridium botulinum types A, B, E and F in honey produced in the Nordic countries.
作者信息
Nevas Mari, Lindström Miia, Hautamäki Kirsi, Puoskari Satu, Korkeala Hannu
机构信息
Department of Food and Environmental Hygiene, Faculty of Veterinary Medicine, University of Helsinki, P.O. Box 66, 00014 Helsinki, Finland.
出版信息
Int J Food Microbiol. 2005 Nov 25;105(2):145-51. doi: 10.1016/j.ijfoodmicro.2005.04.007. Epub 2005 Jul 27.
A total of 294 honey samples produced in Denmark, Norway and Sweden were studied for the presence of Clostridium botulinum types A, B, E and F by using a multiplex-PCR method. The samples consisted of honeycombs taken directly from beehives, and extracted honey representing several hives or apiaries. The prevalence of C. botulinum showed a significant variation between Denmark, Norway and Sweden, the proportions of positive samples being 26%, 10% and 2%, respectively. The major serotype detected was type B. When analysed with pulsed-field gel electrophoresis (PFGE) using restriction enzyme SacII, the 24 strains isolated produced eight different PFGE patterns. At a similarity level of 95%, four clusters were produced, three of which contained 20 of the 24 analysed strains. One of the clusters included isolates from both Denmark and Norway.
采用多重聚合酶链反应（multiplex-PCR）方法，对丹麦、挪威和瑞典生产的总共294份蜂蜜样本进行了肉毒梭菌A、B、E和F型检测。样本包括直接从蜂箱采集的蜂巢，以及代表多个蜂箱或养蜂场的提取蜂蜜。肉毒梭菌的流行率在丹麦、挪威和瑞典之间存在显著差异，阳性样本比例分别为26%、10%和2%。检测到的主要血清型为B型。使用限制性内切酶SacII通过脉冲场凝胶电泳（PFGE）分析时，分离出的24株菌株产生了八种不同的PFGE图谱。在95%的相似性水平下，产生了四个簇，其中三个簇包含24株分析菌株中的20株。其中一个簇包括来自丹麦和挪威的分离株。
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