Nevas Mari, Lindström Miia, Hautamäki Kirsi, Puoskari Satu, Korkeala Hannu
Department of Food and Environmental Hygiene, Faculty of Veterinary Medicine, University of Helsinki, P.O. Box 66, 00014 Helsinki, Finland.
Int J Food Microbiol. 2005 Nov 25;105(2):145-51. doi: 10.1016/j.ijfoodmicro.2005.04.007. Epub 2005 Jul 27.
A total of 294 honey samples produced in Denmark, Norway and Sweden were studied for the presence of Clostridium botulinum types A, B, E and F by using a multiplex-PCR method. The samples consisted of honeycombs taken directly from beehives, and extracted honey representing several hives or apiaries. The prevalence of C. botulinum showed a significant variation between Denmark, Norway and Sweden, the proportions of positive samples being 26%, 10% and 2%, respectively. The major serotype detected was type B. When analysed with pulsed-field gel electrophoresis (PFGE) using restriction enzyme SacII, the 24 strains isolated produced eight different PFGE patterns. At a similarity level of 95%, four clusters were produced, three of which contained 20 of the 24 analysed strains. One of the clusters included isolates from both Denmark and Norway.
采用多重聚合酶链反应（multiplex-PCR）方法，对丹麦、挪威和瑞典生产的总共294份蜂蜜样本进行了肉毒梭菌A、B、E和F型检测。样本包括直接从蜂箱采集的蜂巢，以及代表多个蜂箱或养蜂场的提取蜂蜜。肉毒梭菌的流行率在丹麦、挪威和瑞典之间存在显著差异，阳性样本比例分别为26%、10%和2%。检测到的主要血清型为B型。使用限制性内切酶SacII通过脉冲场凝胶电泳（PFGE）分析时，分离出的24株菌株产生了八种不同的PFGE图谱。在95%的相似性水平下，产生了四个簇，其中三个簇包含24株分析菌株中的20株。其中一个簇包括来自丹麦和挪威的分离株。
