Abdel-Moein Khaled A, Hamza Dalia A
a Faculty of Veterinary Medicine, Department of Zoonoses , Cairo University , Cairo 12211 , Egypt.
Pathog Glob Health. 2016;110(1):25-9. doi: 10.1080/20477724.2015.1133107. Epub 2016 Jan 22.
The current study was conducted to investigate the occurrence of human pathogenic Clostridium botulinum in the feces of dairy animals. Fecal samples were collected from 203 apparently healthy dairy animals (50 cattle, 50 buffaloes, 52 sheep, 51 goats). Samples were cultured to recover C. botulinum while human pathogenic C. botulinum strains were identified after screening of all C. botulinum isolates for the presence of genes that encode toxins type A, B, E, F. The overall prevalence of C. botulinum was 18.7% whereas human pathogenic C. botulinum strains (only type A) were isolated from six animals at the rates of 2, 2, 5.8, and 2% for cattle, buffaloes, sheep, and goats, respectively. High fecal carriage rates of C. botulinum among apparently healthy dairy animals especially type A alarm both veterinary and public health communities for a potential role which may be played by dairy animals in the epidemiology of such pathogen.
本研究旨在调查奶牛粪便中人类致病性肉毒梭菌的存在情况。从203头看似健康的奶牛（50头牛、50头水牛、52只绵羊、51只山羊）采集粪便样本。对样本进行培养以分离肉毒梭菌，同时在筛选所有肉毒梭菌分离株是否存在编码A、B、E、F型毒素的基因后，鉴定人类致病性肉毒梭菌菌株。肉毒梭菌的总体患病率为18.7%，而人类致病性肉毒梭菌菌株（仅A型）从6只动物中分离得到，牛、水牛、绵羊和山羊的分离率分别为2%、2%、5.8%和2%。看似健康的奶牛中肉毒梭菌的高粪便携带率，尤其是A型，引起了兽医和公共卫生界对奶牛在这种病原体流行病学中可能发挥的潜在作用的关注。
