T7病毒早期区域的限制性内切酶切割图谱分析
Restriction enzyme cleavage mapping of T7 virus early region.
作者信息
Gordon R L, Humphries P, McConnell D J
出版信息
Mol Gen Genet. 1978 Jul 4;162(3):329-39. doi: 10.1007/BF00268859.
DNA molecules of seven T7 mutants with overlapping deletions in the early region were cleaved by restriction enzymes HindII, HpaI and II, and HaeIII. The differences in the cleavage patterns after electrophoresis have been used to generate a cleavage map of the restriction sites of this enzyme. It covers the first 9% of the T7 DNA molecule. Cleavage points for HindII are at 0.60, 1.33, 1.59, 1.76, 5.26, 6.27, 7.4 and 8.38%; for HpaI and II at 1.36, 1.62, 4.46, 6.29, 6.62, 7.56, and 8.76%, for HaeIII at 3.85, 6.98, 7.88 and 8.26%. Some fragments have been located in the region containing the early promoters, others carry the complete sequences of gene 0.3.
对七个在早期区域有重叠缺失的T7突变体的DNA分子用限制性内切酶HindII、HpaI和II以及HaeIII进行切割。电泳后切割模式的差异被用于绘制该酶限制性位点的切割图谱。它覆盖了T7 DNA分子的前9%。HindII的切割点分别在0.60%、1.33%、1.59%、1.76%、5.26%、6.27%、7.4%和8.38%处；HpaI和II的切割点分别在1.36%、1.62%、4.46%、6.29%、6.62%、7.56%和8.76%处，HaeIII的切割点分别在3.85%、6.98%、7.88%和8.26%处。一些片段位于包含早期启动子的区域，其他片段则携带基因0.3的完整序列。
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