与半乳糖操纵子结构基因融合的大肠杆菌核糖体RNA启动子的基因表达。

Gene expression of an Escherichia coli ribosomal RNA promoter fused to structural genes of the galactose operon.

作者信息

Ota Y, Kikuchi A, Cashel M

出版信息

Proc Natl Acad Sci U S A. 1979 Nov;76(11):5799-803. doi: 10.1073/pnas.76.11.5799.

DOI:10.1073/pnas.76.11.5799

PMID:160557

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC411738/

Abstract

The promoter region of the rrnB ribosomal RNA gene of Escherichia coli has been ligated within the epimerase gene (galE) of the galactose operon in a lambda phage vector. The recombinant lambda phage has been characterized by restriction mapping and assays of both galK (galactokinase) gene activity and galactose messenger RNA hybridization. In such lyosgens, expression of the fused galactose operon occurs as a function of growth rate in a manner characteristic of ribosomal RNA gene expression and is subject to stringent control by amino acid availability for protein synthesis. Galactose messenger RNA arising from the ribosomal promoter is not as metabolically stable as ribosomal RNA.

摘要

大肠杆菌rrnB核糖体RNA基因的启动子区域已被连接到λ噬菌体载体中半乳糖操纵子的表异构酶基因（galE）内。通过限制性酶切图谱分析以及对galK（半乳糖激酶）基因活性和半乳糖信使RNA杂交的检测，对重组λ噬菌体进行了表征。在这样的溶原菌中，融合的半乳糖操纵子的表达作为生长速率的函数而发生，其方式具有核糖体RNA基因表达的特征，并且受到蛋白质合成中氨基酸可用性的严格控制。源自核糖体启动子的半乳糖信使RNA在代谢上不如核糖体RNA稳定。