Liebke H, Hatfull G
Nucleic Acids Res. 1985 Aug 12;13(15):5515-25. doi: 10.1093/nar/13.15.5515.
The 1440 nucleotides of the distal region of the E. coli ribosomal RNA operon found on the lambda aroE transducing phage has been sequenced. We show that the lambda aroE hybrid rrn operon ends after a solitary 5S RNA gene with rrnE distal sequence. A single terminator structure of dyad symmetry followed by a run of six T's have been identified and compared to other sequenced rrn terminator hairpins. Immediately adjacent to the hairpin is a region of interrupted but conserved sequence that is shared by rrnE, rrnB and rrnD. An open reading frame of 127 amino acids abuts the terminator structure. Another open reading frame of 147 amino acids is found on the opposite strand several hundred nucleotides downstream.
已对λaroE转导噬菌体上发现的大肠杆菌核糖体RNA操纵子远端区域的1440个核苷酸进行了测序。我们发现，λaroE杂交rrn操纵子在带有rrnE远端序列的单个5S RNA基因之后结束。已鉴定出一个具有二元对称性的单一终止子结构，其后跟着一串六个T，并与其他已测序的rrn终止子发夹结构进行了比较。紧邻发夹结构的是一个中断但保守的序列区域，rrnE、rrnB和rrnD共有该区域。一个127个氨基酸的开放阅读框邻接终止子结构。在下游几百个核苷酸处的相反链上发现了另一个147个氨基酸的开放阅读框。
