Chiaramello A E, Zyskind J W
Department of Biology, San Diego State University, California 92182.
J Bacteriol. 1989 Aug;171(8):4272-80. doi: 10.1128/jb.171.8.4272-4280.1989.
The synthesis of specific cellular components related to the initiation process of DNA replication was correlated with changes in growth rate. The concentrations of DnaA protein and mioC mRNA were determined for cells grown at six different growth rates; both increased relative to either total protein or total RNA, respectively, as the growth rate increased. Expression from the chromosomal mioC promoter, which contains a DnaA protein-binding site, was not repressed when the DnaA protein concentration was increased and was not derepressed in a dnaA46 mutant at 42 degrees C. The mioC transcript had a characteristic mRNA-type half-life of 1.51 min.
与DNA复制起始过程相关的特定细胞成分的合成与生长速率的变化相关。测定了在六种不同生长速率下生长的细胞中DnaA蛋白和mioC mRNA的浓度；随着生长速率的增加，它们分别相对于总蛋白或总RNA增加。含有DnaA蛋白结合位点的染色体mioC启动子的表达在DnaA蛋白浓度增加时未被抑制，并且在42℃的dnaA46突变体中也未被去抑制。mioC转录本具有1.51分钟的特征性mRNA型半衰期。
