苏云金芽孢杆菌Cry4Ba和Cyt2Aa2在大肠杆菌中的共表达显示出对埃及伊蚊和致倦库蚊幼虫的高度协同作用。
Co-expression of Bacillus thuringiensis Cry4Ba and Cyt2Aa2 in Escherichia coli revealed high synergism against Aedes aegypti and Culex quinquefasciatus larvae.
作者信息
Promdonkoy Boonhiang, Promdonkoy Patcharee, Panyim Sakol
机构信息
National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Paholyothin Road, Klong 1, Klong Luang, Pathumthani 12120, Thailand.
出版信息
FEMS Microbiol Lett. 2005 Nov 1;252(1):121-6. doi: 10.1016/j.femsle.2005.08.038. Epub 2005 Sep 7.
Cry4Ba is a delta-endotoxin produced by Bacillus thuringiensis subsp. israelensis and Cyt2Aa2 is a cytolytic delta-endotoxin produced by B. thuringiensis subsp. darmstadiensis. Cry4Ba produced in Escherichia coli was toxic to Aedes aegypti larvae (LC(50)=140 ng ml(-1)) but virtually inactive to Culex quinquefasciatus larvae. Cyt2Aa2 expressed in E. coli exhibited moderate activity against A. aegypti and C. quinquefasciatus larvae with LC(50) values of 350 and 250 ng ml(-1), respectively. Co-expression of both toxins in E. coli dramatically increased toxicity to both A. aegypti andC. quinquefasciatus larvae (LC(50)=7 and 20 ng ml(-1), respectively). This is the first report to demonstrate that Cry4Ba and Cyt2Aa2 have high synergistic activity against C. quinquefasciatus larvae.
Cry4Ba是苏云金芽孢杆菌以色列亚种产生的一种δ-内毒素，而Cyt2Aa2是苏云金芽孢杆菌达姆施塔特亚种产生的一种溶细胞性δ-内毒素。在大肠杆菌中产生的Cry4Ba对埃及伊蚊幼虫有毒性（半数致死浓度[LC(50)] = 140纳克/毫升），但对致倦库蚊幼虫几乎无活性。在大肠杆菌中表达的Cyt2Aa2对埃及伊蚊和致倦库蚊幼虫表现出中等活性，其LC(50)值分别为350和250纳克/毫升。两种毒素在大肠杆菌中的共表达显著增加了对埃及伊蚊和致倦库蚊幼虫的毒性（LC(50)分别为7和20纳克/毫升）。这是首次证明Cry4Ba和Cyt2Aa2对致倦库蚊幼虫具有高协同活性的报告。
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