Department of Biology, Faculty of Science and Art, Giresun University, 28200, Giresun, Turkey.
Sci Rep. 2021 Sep 10;11(1):18081. doi: 10.1038/s41598-021-95879-7.
In this study, the toxic effects of aflatoxin B (AFB) on Swiss albino mice and the protective effects of resveratrol were investigated. Physiological (body weight, liver and kidney weight), biochemical (aspartate aminotransferase-AST, alanine transaminase-ALT, blood urea nitrogen-BUN, creatinine, malondialdehyde-MDA and glutathione-GSH) and cytogenetic parameters (micronucleus-MN in buccal epithelium, erythrocyte and leukocyte cells and chromosomal aberrations-CAs) were used to determine the toxic effects. Additionally, scavenging effects of resveratrol against superoxide, hydrogen peroxide (HO) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals were also investigated. In experimental period, mice were divided into six groups and the groups were treated with tap water, 10 mg/kg b.w resveratrol, 20 mg/kg b.w resveratrol, 20 µg/kg b.w. AFB, 10 mg/kg b.w resveratrol + 20 µg/kg b.w AFB, 20 mg/kg b.w resveratrol + 20 µg/kg b.w AFB, respectively. As a result, the scavenging effects of resveratrol increased with increasing dose and the superoxide, HO and DPPH radical scavenging activity of resveratrol were 74.9%, 79.1% and 49.2%, respectively. AFB administration caused a significant decrease in physiological parameters, and these decreases regressed in AFB + resveratrol treated groups. Serum ALT and AST activities, BUN and creatinine levels were higher in the AFB treated group compared to the control group and serious abnormalities were found in MDA and GSH levels in the kidney and liver. In the group treated with AFB + 20 mg/kg resveratrol, ALT, AST, BUN and creatinine levels decreased significantly and GSH levels increased compared to only-AFB treated group. AFB triggered MN formation in buccal epithelium, erythrocyte and leukocyte cells and CAs in bone marrow cells. The application of 20 mg/kg resveratrol together with AFB was decreased the MN and CAs frequency. Resveratrol exhibited a recovery effect in the range of 40.9-80.5% against AFB toxicity in all tested parameters. In this study, it was determined that AFB caused serious changes in selected physiological, biochemical and cytogenetic parameters while resveratrol displayed a protective role against these toxic effects.
本研究旨在探讨黄曲霉毒素 B（AFB）对瑞士白化小鼠的毒性作用以及白藜芦醇的保护作用。通过生理（体重、肝重和肾重）、生化（天门冬氨酸氨基转移酶-AST、丙氨酸氨基转移酶-ALT、血尿素氮-BUN、肌酐、丙二醛-MDA 和谷胱甘肽-GSH）和细胞遗传学参数（口腔上皮细胞、红细胞和白细胞中的微核-MN 以及骨髓细胞中的染色体畸变-CAs）来确定毒性作用。此外，还研究了白藜芦醇对超氧自由基、过氧化氢（HO）和 1,1-二苯基-2-苦基肼基（DPPH）自由基的清除作用。在实验期间，将小鼠分为六组，分别用自来水、10mg/kg b.w 白藜芦醇、20mg/kg b.w 白藜芦醇、20μg/kg b.w AFB、10mg/kg b.w 白藜芦醇+20μg/kg b.w AFB、20mg/kg b.w 白藜芦醇+20μg/kg b.w AFB 进行处理。结果表明，白藜芦醇的清除作用随剂量的增加而增强，其对超氧自由基、HO 和 DPPH 自由基的清除活性分别为 74.9%、79.1%和 49.2%。AFB 给药导致生理参数显著下降，而在 AFB+白藜芦醇处理组中这些下降得到恢复。与对照组相比，AFB 处理组的血清 ALT 和 AST 活性、BUN 和肌酐水平升高，并且在肾脏和肝脏中 MDA 和 GSH 水平出现严重异常。在仅用 AFB 处理组中，20mg/kg 白藜芦醇处理组的 ALT、AST、BUN 和肌酐水平显著降低，GSH 水平升高。AFB 诱导口腔上皮细胞、红细胞和白细胞中的 MN 形成以及骨髓细胞中的 CAs。应用 20mg/kg 白藜芦醇与 AFB 一起处理，MN 和 CAs 的频率降低。白藜芦醇在所有测试参数中对 AFB 毒性表现出 40.9-80.5%的恢复作用。在这项研究中，确定 AFB 导致所选生理、生化和细胞遗传学参数发生严重变化，而白藜芦醇对这些毒性作用表现出保护作用。
