Johnsen H E, Madsen M
Scand J Immunol. 1979;10(3):241-9. doi: 10.1111/j.1365-3083.1979.tb01346.x.
The enhanced stimulation of human B lymphocytes by pokeweed mitogen in the presence of irradiated T helper lymphocytes has been studied, revealing that the proliferative responses measured by incorporation of thymidine in cultures of B lymphocytes and irradiated T lymphocytes in a 1:2 or 1:4 ratio is mostly a function of the B cells. Only a minimal number, if any, of the T cells contaminating the B cell suspensions is stimulated to proliferation. This is in contrast to stimulation of the B-cell suspensions without addition of irradiated T cells, where both B cells and T cells proliferate. The irradiated T helper cells have no FcR for antigen-bound IgG, and as well allogeneic as autologous T cells exhibit helper capacity of equal strength. The test system described makes it possible selectively to test one B-cell function and the corresponding T helper capacity.
研究了在经辐照的T辅助淋巴细胞存在的情况下商陆丝裂原对人B淋巴细胞的增强刺激作用，结果显示，通过在B淋巴细胞与经辐照的T淋巴细胞按1:2或1:4比例培养的体系中掺入胸苷来测量的增殖反应主要是B细胞的功能。污染B细胞悬液的T细胞即使有，也只有极少数被刺激增殖。这与不添加经辐照的T细胞时对B细胞悬液的刺激情况形成对比，在后者中B细胞和T细胞都会增殖。经辐照的T辅助细胞没有针对与抗原结合的IgG的FcR，并且同种异体和自体T细胞均表现出同等强度的辅助能力。所描述的测试系统能够选择性地测试一种B细胞功能以及相应的T辅助能力。
