Johnsen H E, Madsen M, Kristensen T
Scand J Immunol. 1979;10(3):251-5. doi: 10.1111/j.1365-3083.1979.tb01347.x.
The in vitro polyclonal pokeweed mitogen (PWM)-induced activation of human B lymphocytes is enhanced by addition of autologous or allogeneic irradiated T cells. This model for B/T-cell cooperation may be used to define and describe the balance between T helper and T suppressor phenomena. The present study investigates the helper and suppressor capacities of mononuclear cells isolated from peripheral blood of infectious mononucleosis patients during the acute disease and the reconvalescence period. During the acute disease we found a functional lack of T helper capacity; furthermore, the T cells were able to suppress the PWM and T-cell-dependent B-cell proliferation of healthy donor cells. The suppression was non-cytotoxic; i.e. not due to destruction of the responder cells. This phenomenon of non-cytotoxic suppression was found for all seven patients studied and disappeared during the reconvalescence period, indicating that the T lymphocytosis seen in infectious mononucleosis includes an expansion of T suppressor cells.
通过添加自体或异体照射的T细胞，可增强体外多克隆商陆有丝分裂原（PWM）诱导的人B淋巴细胞活化。这种B/T细胞合作模型可用于定义和描述T辅助细胞与T抑制细胞现象之间的平衡。本研究调查了在急性疾病期和恢复期从传染性单核细胞增多症患者外周血中分离出的单核细胞的辅助和抑制能力。在急性疾病期，我们发现T辅助细胞功能缺失；此外，T细胞能够抑制健康供体细胞的PWM和T细胞依赖性B细胞增殖。这种抑制作用无细胞毒性；即不是由于反应细胞的破坏所致。在所研究的所有7例患者中均发现了这种无细胞毒性抑制现象，且在恢复期消失，这表明传染性单核细胞增多症中出现的T淋巴细胞增多包括T抑制细胞的扩增。
