Zografos-Miller L E, Argyris B F
Transplantation. 1983 Jun;35(6):593-600. doi: 10.1097/00007890-198306000-00015.
Suppressor cell activity from P815-allosensitized C57BL/6 spleen can be decreased by incubating the tumor-allosensitized spleen cells on monolayers of thioglycollate-stimulated BDF1 peritoneal macrophages for 2 or 4 hr. The adsorption response appears to be specific for macrophages, because adsorption of suppressor cell activity does not occur following incubation of P815-allosensitized spleen cells on confluent monolayers of mouse spleen cells or mouse embryonic fibroblasts. Pretreatment of macrophage monolayers with X irradiation (2,000 rads) or anti-Thy 1.2 serum (and complement) does not affect their ability to bind suppressor cell activity. Adsorption of suppressor cell activity from P815-allosensitized spleen can also be carried out by proteose peptone-stimulated or Corynebacterium parvum-stimulated macrophages. Blockage of macrophage Fc receptors decreases the ability of thioglycollate-stimulated macrophages to adsorb suppressor cell activity. Monolayers of P815 or P388 cells, two cell types positive for Fc receptors, are unable to adsorb suppressor cell activity from the tumor-allosensitized spleen. The significance of our findings is discussed in terms of the relationship between macrophages and suppressor cells in the immune response to normal or tumor allografts.
将P815同种致敏的C57BL/6脾细胞在巯基乙酸盐刺激的BDF1腹腔巨噬细胞单层上孵育2或4小时，可降低来自P815同种致敏C57BL/6脾的抑制细胞活性。这种吸附反应似乎对巨噬细胞具有特异性，因为将P815同种致敏脾细胞在汇合的小鼠脾细胞单层或小鼠胚胎成纤维细胞上孵育后，不会发生抑制细胞活性的吸附。用X射线照射（2000拉德）或抗Thy 1.2血清（及补体）预处理巨噬细胞单层，并不影响其结合抑制细胞活性的能力。用蛋白胨刺激或微小棒状杆菌刺激的巨噬细胞也能吸附来自P815同种致敏脾的抑制细胞活性。阻断巨噬细胞Fc受体可降低巯基乙酸盐刺激的巨噬细胞吸附抑制细胞活性的能力。P815或P388细胞（两种Fc受体阳性的细胞类型）的单层不能吸附来自肿瘤同种致敏脾的抑制细胞活性。我们根据巨噬细胞与抑制细胞在对正常或肿瘤同种异体移植物的免疫反应中的关系，讨论了我们研究结果的意义。
