Iacopetta B J, Grieu F, Horisberger M, Sunahara G I
Nestlé Research Centre, Lausanne, Switzerland.
Acta Paediatr. 1992 Apr;81(4):287-91. doi: 10.1111/j.1651-2227.1992.tb12227.x.
The concentration of epidermal growth factor in human and bovine milk was measured by radioreceptor assay. Both human placental plasma membranes and a human epidermoid carcinoma cell were used as the epidermal growth factor receptor source in the assay. The use of placental plasma membrane in the radioreceptor assay gave erroneous results for bovine milk and overestimated the concentration of epidermal growth factor in human milk. Intact cells appear to provide a more accurate measure of the concentration of epidermal growth factor in milk samples. Using A431 cells, we found very low concentrations of epidermal growth factor in bovine milk (less than 2 ng/ml) compared to human milk (30-40 ng/ml). No epidermal growth factor activity was found in several cows' milk-based infant formulas. These results highlight the caution which must be taken when measuring trace substances such as polypeptide growth factors in complex samples such as milk.
采用放射受体分析法测定了人乳和牛乳中表皮生长因子的浓度。在该分析中，人胎盘质膜和人表皮样癌细胞均被用作表皮生长因子受体源。在放射受体分析中使用胎盘质膜对牛乳得出了错误结果，并高估了人乳中表皮生长因子的浓度。完整细胞似乎能更准确地测定乳样中表皮生长因子的浓度。使用A431细胞，我们发现与母乳（30 - 40纳克/毫升）相比，牛乳中表皮生长因子的浓度非常低（低于2纳克/毫升）。在几种以牛奶为基础的婴儿配方奶粉中未发现表皮生长因子活性。这些结果凸显了在诸如牛奶等复杂样品中测量微量物质（如多肽生长因子）时必须谨慎。
