Stollar B D, Borel Y
Department of Biochemistry and Pharmacology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.
Nature. 1977 May 12;267(5607):158-60. doi: 10.1038/267158a0.
Since its original development by Jerne, the haemolytic plaque assay has increased our understanding of antibody formation to a wide variety of antigens, including proteins, lipopolysaccharides, and simple haptens. We have now developed an assay to detect plaque forming cells (PFC) making anti-nucleoside antibodies. Previously we reported the suppression of circulating antibody to DNA determinants by nucleoside-IgG conjugates. Here we show that BALB/c mice can be rendered tolerant in terms of both direct and indirect anti-nucleoside antibody forming cells and that the state of tolerance is nucleoside-specific at the cellular level.
自耶尔恩最初开发溶血空斑试验以来，该试验增进了我们对针对多种抗原（包括蛋白质、脂多糖和简单半抗原）抗体形成的理解。我们现已开发出一种检测产生抗核苷抗体的空斑形成细胞（PFC）的试验。此前我们报道了核苷 - IgG 偶联物对循环中针对 DNA 决定簇抗体的抑制作用。在此我们表明，BALB/c 小鼠在直接和间接抗核苷抗体形成细胞方面均可产生耐受性，且这种耐受状态在细胞水平上是核苷特异性的。
