Ohgushi H, Okumura M, Yoshikawa T, Inoue K, Senpuku N, Tamai S, Shors E C
Department of Orthopedics, Nara Medical University, Japan.
J Biomed Mater Res. 1992 Jul;26(7):885-95. doi: 10.1002/jbm.820260705.
This study determined the bone formation in porous calcium carbonate (CC) and porous hydroxyapatite (HA) in ectopic sites. The bone formation stimulus was derived from bone marrow cells. CC and HA in the shape of disks were implanted with or without rat marrow cells into subcutaneous sites of syngeneic rats. The CC and HA had identical microstructure: pore size was 190-230 microns, porosity was 50-60% and they were fully interconnected. Bone did not form in any implants without marrow cells (disks themselves), whereas bone consistently formed in the pores of all implants with marrow cells after 4 weeks. The bone formation of both CC and HA occurred initially on surface of the pore regions and progressed toward the center of the pore. Scanning electron microscopy and electron-probe microanalysis revealed a continuum of calcium at the interfaces of both bone/CC and bone/HA implants. These results indicate that the bone formation in calcium carbonate derived from marine corals is comparable to the bioactive hydroxyapatite.
本研究确定了异位部位多孔碳酸钙（CC）和多孔羟基磷灰石（HA）中的骨形成情况。骨形成刺激源自骨髓细胞。将圆盘状的CC和HA与大鼠骨髓细胞一起或不与大鼠骨髓细胞一起植入同基因大鼠的皮下部位。CC和HA具有相同的微观结构：孔径为190 - 230微米，孔隙率为50 - 60%，且它们完全相互连通。在没有骨髓细胞的任何植入物（圆盘本身）中均未形成骨，而在4周后，所有带有骨髓细胞的植入物的孔隙中均持续形成了骨。CC和HA的骨形成最初都发生在孔隙区域的表面，并向孔隙中心发展。扫描电子显微镜和电子探针微分析显示，在骨/CC和骨/HA植入物的界面处钙是连续的。这些结果表明，源自海洋珊瑚的碳酸钙中的骨形成与生物活性羟基磷灰石相当。
