Loening Nikolaus M, Chamberlin Anne M, Zepeda Andrea G, Gonzalez R Gilberto, Cheng Leo L
Department of Chemistry, Lewis & Clark College, Portland, OR 97219, USA.
NMR Biomed. 2005 Nov;18(7):413-20. doi: 10.1002/nbm.973.
Choline and the related compounds phosphocholine (PC) and glycerophosphocholine (GPC) are considered to be important metabolites in oncology. Past studies have demonstrated correlations linking the relative ratios and concentrations of these metabolites with the development and progression of cancer. Currently, in vivo and tissue ex vivo magnetic resonance spectroscopy methods have mostly centered on measuring the total concentration of these metabolites and have difficulty in differentiating between them. Here, a new scheme that uses (31)P edited (1)H spectroscopy to quantify the concentrations of choline, PC and GPC in biological samples is reported and its applicability is demonstrated using samples of human brain tumor extracts. This method is particularly well-suited for analytical situations where the PC and GPC resonances are not sufficiently resolved and/or are obscured by other metabolites. Consequently, this scheme has the potential to be used for the analysis of choline compounds in ex vivo tissue samples.
胆碱以及相关化合物磷酸胆碱（PC）和甘油磷酸胆碱（GPC）被认为是肿瘤学中的重要代谢产物。过去的研究表明，这些代谢产物的相对比例和浓度与癌症的发生和发展之间存在关联。目前，体内和组织离体磁共振波谱法主要集中于测量这些代谢产物的总浓度，并且难以区分它们。在此，报告了一种使用（31）P编辑（1）H波谱法来定量生物样品中胆碱、PC和GPC浓度的新方案，并使用人脑肿瘤提取物样品证明了其适用性。该方法特别适用于PC和GPC共振未得到充分分辨和/或被其他代谢产物掩盖的分析情况。因此，该方案有潜力用于离体组织样品中胆碱化合物的分析。
