Mohd-Sarip Adone, Cléard Fabienne, Mishra Rakesh K, Karch François, Verrijzer C Peter
Department of Biochemistry, Centre for Biomedical Genetics, Erasmus University Medical Center, 3000 DR Rotterdam, The Netherlands.
Genes Dev. 2005 Aug 1;19(15):1755-60. doi: 10.1101/gad.347005.
Polycomb response elements (PREs) are cis-acting DNA elements that mediate epigenetic gene silencing by Polycomb group (PcG) proteins. Here, we report that Pleiohomeotic (PHO) and a multiprotein Polycomb core complex (PCC) bind highly cooperatively to PREs. We identified a conserved sequence motif, named PCC-binding element (PBE), which is required for PcG silencing in vivo. PHO sites and PBEs function as an integrated DNA platform for the synergistic assembly of a repressive PHO/PCC complex. We termed this nucleoprotein complex silenceosome to reflect that the molecular principles underpinning its assemblage are surprisingly similar to those that make an enhanceosome.
多梳应答元件（PREs）是顺式作用的DNA元件，可介导多梳蛋白家族（PcG）蛋白对基因进行表观遗传沉默。在此，我们报告称，多梳抑制蛋白（PHO）和一种多蛋白多梳核心复合体（PCC）与PREs高度协同结合。我们鉴定出一个保守的序列基序，命名为PCC结合元件（PBE），它是体内PcG沉默所必需的。PHO位点和PBE作为一个整合的DNA平台，用于协同组装抑制性PHO/PCC复合体。我们将这种核蛋白复合体称为沉默体，以反映其组装的分子原理与增强体的分子原理惊人地相似。
