Kobayashi H, Ida W, Terao T, Kawashima Y
Department of Obstetrics and Gynecology, Hamamatsu University School of Medicine, Shizuoka, Japan.
Int J Cancer. 1993 May 28;54(3):426-34. doi: 10.1002/ijc.2910540313.
We have established an in vitro system for the culture of epithelial cells (ECs) from human uterine endometrium to examine the production of CA 125 and to characterize the CA-125 antigen purified from the conditioned media. CA-125 secretion was higher in heterotopic ECs than in eutopic ECs and it was more significant after heterotopic ECs reached confluence than during the logarithmic growth phase. CA-125 expression was observed mainly in the G0/G1-phase. CA-125 expression on cell membranes did not correlate with the volume of CA 125 released per cell, and there was no amplification of CA-125 expression in heterotopic EC membranes. Treatment of the purified CA-125 antigen with 6 M urea yielded a much lower molecular-mass peak (200 kDa). The results of Western blot indicated the presence of a single polydisperse band of 200 kDa in the conditioned media from eutopic ECs, whereas 2 major CA-125 isoforms of 200 kDa and 110 kDa, as well as 2 minor forms of 100 kDa and 70 kDa, were observed in the conditioned media of the heterotopic ECs. We conclude that, in heterotopic ECs, the 110-kDa CA-125 is more prominent than the 200-kDa antigen, and that the elevation of CA-125 levels in the conditioned media could be attributed to significantly increased release of 110-kDa CA-125 from heterotopic ECs.
我们建立了一种体外培养人子宫内膜上皮细胞（ECs）的系统，用于检测CA 125的产生，并对从条件培养基中纯化的CA - 125抗原进行特性分析。异位ECs中CA - 125的分泌高于在位ECs，且异位ECs汇合后比对数生长期时更为显著。CA - 125表达主要出现在G0/G1期。细胞膜上的CA - 125表达与每个细胞释放的CA 125量无关，异位EC膜中CA - 125表达也没有扩增。用6 M尿素处理纯化的CA - 125抗原产生了一个分子量低得多的峰（200 kDa）。蛋白质印迹结果表明，在位ECs的条件培养基中存在一条200 kDa的单一多分散条带，而异位ECs的条件培养基中观察到200 kDa和110 kDa的2种主要CA - 125异构体，以及100 kDa和70 kDa的2种次要形式。我们得出结论，在异位ECs中，110 kDa的CA - 125比200 kDa的抗原更突出，条件培养基中CA - 125水平的升高可能归因于异位ECs中110 kDa CA - 125释放的显著增加。
