Kim Yu-Jin, Oh You-Kwan, Kang Whankoo, Lee Eun Yeol, Park Sunghoon
Department of Chemical Engineering, Pusan National University, 30 Jangjeon-dong, Geumjeong-gu, Busan, 609-735, Republic of Korea.
J Ind Microbiol Biotechnol. 2005 Sep;32(9):402-8. doi: 10.1007/s10295-005-0010-2. Epub 2005 Nov 3.
Caseinomacropeptide is a polypeptide of 64 amino acid residues (106-169) derived from the C-terminal part of the mammalian milk k-casein. This macropeptide has various biological activities and is used as a functional food ingredient as well as a pharmaceutical compound. The gene encoding the human caseinomacropeptide (hCMP) was synthesized and expressed with an alpha-factor secretion signal in the two yeast strains, Saccharomyces cerevisiae and Pichia pastoris. The complete polypeptide of the recombinant hCMP was produced and secreted in a culture medium by both the strains, but the highest production was observed in S. cerevisiae with a galactose-inducible promoter. In a fed-batch bioreactor culture, 2.5 g/l of the recombinant hCMP was obtained from the S. cerevisiae at 97 h.
酪蛋白巨肽是一种由64个氨基酸残基（106 - 169）组成的多肽，来源于哺乳动物乳中κ-酪蛋白的C末端部分。这种巨肽具有多种生物活性，被用作功能性食品成分以及药物化合物。编码人酪蛋白巨肽（hCMP）的基因在酿酒酵母和巴斯德毕赤酵母这两种酵母菌株中，与α-因子分泌信号一起进行合成和表达。两种菌株都在培养基中产生并分泌了重组hCMP的完整多肽，但在具有半乳糖诱导型启动子的酿酒酵母中产量最高。在补料分批生物反应器培养中，97小时时从酿酒酵母中获得了2.5 g/l的重组hCMP。
