Regulated GAL4 expression cassette providing controllable and high-level output from high-copy galactose promoters in yeast.

Suppr

超能文献

作者信息

Mylin L M, Hofmann K J, Schultz L D, Hopper J E

出版信息

Methods Enzymol. 1990;185:297-308. doi: 10.1016/0076-6879(90)85026-k.

DOI:10.1016/0076-6879(90)85026-k

PMID:2199783

Abstract

摘要

相似文献

Regulated GAL4 expression cassette providing controllable and high-level output from high-copy galactose promoters in yeast.

Methods Enzymol. 1990;185:297-308. doi: 10.1016/0076-6879(90)85026-k.

Expression of the transcriptional activator LAC9 (KlGAL4) in Kluyveromyces lactis is controlled by autoregulation.转录激活因子LAC9（KlGAL4）在乳酸克鲁维酵母中的表达受自身调控。

Mol Cell Biol. 1993 May;13(5):3058-66. doi: 10.1128/mcb.13.5.3058-3066.1993.

Catabolite repression by galactose in overexpressed GAL4 strains of Saccharomyces cerevisiae.在酿酒酵母中过表达GAL4的菌株中半乳糖介导的分解代谢物阻遏作用

J Gen Microbiol. 1991 May;137(5):1039-44. doi: 10.1099/00221287-137-5-1039.

The overexpression of the CDC25 gene of Saccharomyces cerevisiae causes a derepression of GAL system and an increase of GAL4 transcription.酿酒酵母CDC25基因的过表达会导致GAL系统的去阻遏以及GAL4转录的增加。

Int J Biochem Cell Biol. 2000 Feb;32(2):215-24. doi: 10.1016/s1357-2725(99)00121-1.

Inducible expression cassettes in yeast: GAL4.酵母中的可诱导表达盒：GAL4

Methods Mol Biol. 1997;62:131-48. doi: 10.1385/0-89603-480-1:131.

A new class of yeast transcriptional activators.一类新型的酵母转录激活因子。

Cell. 1987 Oct 9;51(1):113-9. doi: 10.1016/0092-8674(87)90015-8.

Vectors allowing amplified expression of the Saccharomyces cerevisiae Gal3p-Gal80p-Gal4p transcription switch: applications to galactose-regulated high-level production of proteins.能够增强酿酒酵母Gal3p-Gal80p-Gal4p转录开关表达的载体：在半乳糖调控的蛋白质高水平生产中的应用

Protein Expr Purif. 2000 Mar;18(2):202-12. doi: 10.1006/prep.1999.1177.

High-throughput screening for protein-protein interactions using two-hybrid assay.使用双杂交测定法进行蛋白质-蛋白质相互作用的高通量筛选。

Methods Enzymol. 2000;328:3-14. doi: 10.1016/s0076-6879(00)28386-9.

Transcriptional control of yeast phosphoglycerate mutase-encoding gene.酵母磷酸甘油酸变位酶编码基因的转录调控

Gene. 1993 Mar 30;125(2):125-33. doi: 10.1016/0378-1119(93)90319-x.

Ets1, when fused to the GAL4 DNA binding domain, efficiently enhances galactose promotor dependent gene expression in yeast.当Ets1与GAL4 DNA结合结构域融合时，它能有效增强酵母中半乳糖启动子依赖性基因的表达。

Oncogene. 1991 Mar;6(3):357-60.

引用本文的文献

Purification of transmembrane proteins from Saccharomyces cerevisiae for X-ray crystallography.从酿酒酵母中纯化跨膜蛋白用于X射线晶体学研究。

Protein Expr Purif. 2010 Jun;71(2):207-23. doi: 10.1016/j.pep.2009.12.012. Epub 2010 Jan 4.

Targeted activation of transcription in vivo through hairpin-triplex forming oligonucleotide in Saccharomyces cerevisiae.通过发夹-三链体形成寡核苷酸在酿酒酵母中实现体内转录的靶向激活。

Mol Cell Biochem. 2005 Oct;278(1-2):147-55. doi: 10.1007/s11010-005-7283-7.

Production of human caseinomacropeptide in recombinant Saccharomyces cerevisiae and Pichia pastoris.在重组酿酒酵母和巴斯德毕赤酵母中生产人酪蛋白巨肽。

J Ind Microbiol Biotechnol. 2005 Sep;32(9):402-8. doi: 10.1007/s10295-005-0010-2. Epub 2005 Nov 3.

Cloning and molecular characterization of the delta6-desaturase from two echium plant species: production of GLA by heterologous expression in yeast and tobacco.两种蓝蓟属植物中Δ6-去饱和酶的克隆及分子特性：通过在酵母和烟草中的异源表达生产γ-亚麻酸

Lipids. 2002 Apr;37(4):417-26. doi: 10.1007/s1145-002-0910-6.

The Gal3p-Gal80p-Gal4p transcription switch of yeast: Gal3p destabilizes the Gal80p-Gal4p complex in response to galactose and ATP.酵母的Gal3p-Gal80p-Gal4p转录开关：Gal3p在半乳糖和ATP存在时使Gal80p-Gal4p复合物不稳定。

Mol Cell Biol. 1999 Nov;19(11):7828-40. doi: 10.1128/MCB.19.11.7828.

Novel Gal3 proteins showing altered Gal80p binding cause constitutive transcription of Gal4p-activated genes in Saccharomyces cerevisiae.显示Gal80p结合改变的新型Gal3蛋白导致酿酒酵母中Gal4p激活基因的组成型转录。

Mol Cell Biol. 1997 May;17(5):2566-75. doi: 10.1128/MCB.17.5.2566.

Construction of the complete rat fatty acid synthase cDNA and its expression in Saccharomyces cerevisiae.大鼠脂肪酸合酶全长cDNA的构建及其在酿酒酵母中的表达。

Curr Genet. 1996 Feb;29(3):219-26. doi: 10.1007/BF02221551.

Optimization of the expression system using galactose-inducible promoter for the production of anticoagulant hirudin in Saccharomyces cerevisiae.利用半乳糖诱导型启动子优化酿酒酵母中抗凝水蛭素生产的表达系统。

Appl Microbiol Biotechnol. 1994 Dec;42(4):587-94. doi: 10.1007/BF00173925.

Purification and binding properties of the Mal63p activator of Saccharomyces cerevisiae.

Curr Genet. 1995 May;27(6):509-16. doi: 10.1007/BF00314440.

Phosphorylated forms of GAL4 are correlated with ability to activate transcription.GAL4的磷酸化形式与激活转录的能力相关。

Mol Cell Biol. 1990 Sep;10(9):4623-9. doi: 10.1128/mcb.10.9.4623-4629.1990.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型，支持多种主流文档格式。

立即体验