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Regulated GAL4 expression cassette providing controllable and high-level output from high-copy galactose promoters in yeast.

作者信息

Mylin L M, Hofmann K J, Schultz L D, Hopper J E

出版信息

Methods Enzymol. 1990;185:297-308. doi: 10.1016/0076-6879(90)85026-k.

DOI:10.1016/0076-6879(90)85026-k
PMID:2199783
Abstract
摘要

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1
Regulated GAL4 expression cassette providing controllable and high-level output from high-copy galactose promoters in yeast.
Methods Enzymol. 1990;185:297-308. doi: 10.1016/0076-6879(90)85026-k.
2
Expression of the transcriptional activator LAC9 (KlGAL4) in Kluyveromyces lactis is controlled by autoregulation.转录激活因子LAC9（KlGAL4）在乳酸克鲁维酵母中的表达受自身调控。
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Catabolite repression by galactose in overexpressed GAL4 strains of Saccharomyces cerevisiae.在酿酒酵母中过表达GAL4的菌株中半乳糖介导的分解代谢物阻遏作用
J Gen Microbiol. 1991 May;137(5):1039-44. doi: 10.1099/00221287-137-5-1039.
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The overexpression of the CDC25 gene of Saccharomyces cerevisiae causes a derepression of GAL system and an increase of GAL4 transcription.酿酒酵母CDC25基因的过表达会导致GAL系统的去阻遏以及GAL4转录的增加。
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Inducible expression cassettes in yeast: GAL4.酵母中的可诱导表达盒：GAL4
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6
A new class of yeast transcriptional activators.一类新型的酵母转录激活因子。
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7
Vectors allowing amplified expression of the Saccharomyces cerevisiae Gal3p-Gal80p-Gal4p transcription switch: applications to galactose-regulated high-level production of proteins.能够增强酿酒酵母Gal3p-Gal80p-Gal4p转录开关表达的载体：在半乳糖调控的蛋白质高水平生产中的应用
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High-throughput screening for protein-protein interactions using two-hybrid assay.使用双杂交测定法进行蛋白质-蛋白质相互作用的高通量筛选。
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Transcriptional control of yeast phosphoglycerate mutase-encoding gene.酵母磷酸甘油酸变位酶编码基因的转录调控
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Ets1, when fused to the GAL4 DNA binding domain, efficiently enhances galactose promotor dependent gene expression in yeast.当Ets1与GAL4 DNA结合结构域融合时，它能有效增强酵母中半乳糖启动子依赖性基因的表达。
Oncogene. 1991 Mar;6(3):357-60.

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