[去神经支配诱导大鼠睾丸组织中Bax和Bcl-2基因的表达]
[Expression of Bax and Bcl-2 gene in rat testis tissues induced by testicular denervation].
作者信息
Gong Yong-guang, Yang Yu-ru, Zhagn Wei, Gu Min, Yin Chang-jun
机构信息
Department of Urology, First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, 210029 China.
出版信息
Zhonghua Nan Ke Xue. 2006 Dec;12(12):1063-5.
OBJECTIVE
To detect the changes of the expression of Bax and Bcl-2 gene in the denervated testis, and to explore the possible mechanisms underlying the apoptosis of germ cells induced by testicular denervation at the genetic translation level.
METHODS
Eighteen mature SD rats (350-375 g) were equally divided into 3 groups: a sham operation group( SO) , a superior spermatic nerve group (SSN) and an inferior spermatic nerve group (ISN) , and the latter two received bilateral surgical removal of the superior spermatic nerve and the inferior spermatic nerve, respectively. The animals were killed I month after the operation. ISH SP-method was used to detect the expression of Bax and Bcl-2 protein.
RESULTS
Significant up-regulation of Bax protein was detected in both the treatment groups 1 month after surgery( P <0. 05) , and the level of Bcl-2 protein remained unchanged.
CONCLUSION
Bax gene is involved in the apoptosis of germ cells induced by testicular denervation.
目的
检测去神经支配睾丸中Bax和Bcl-2基因表达的变化，从基因翻译水平探讨睾丸去神经支配诱导生殖细胞凋亡的可能机制。
方法
18只成熟SD大鼠（350 - 375 g）平均分为3组：假手术组（SO）、精索上神经组（SSN）和精索下神经组（ISN），后两组分别接受双侧精索上神经和精索下神经手术切除。术后1个月处死动物。采用ISH SP法检测Bax和Bcl-2蛋白的表达。
结果
术后1个月，两个治疗组中Bax蛋白均显著上调（P < 0.05），而Bcl-2蛋白水平保持不变。
结论
Bax基因参与睾丸去神经支配诱导的生殖细胞凋亡。
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