Yozu Masato, Tabata Hidenori, Nakajima Kazunori
Department of Anatomy, Keio University School of Medicine, Tokyo 160-8582, Japan.
J Neurosci. 2005 Aug 3;25(31):7268-77. doi: 10.1523/JNEUROSCI.2072-05.2005.
The migratory paths of interneurons derived from the ganglionic eminence (GE), and particularly its caudal portion (CGE), remain essentially unknown. To clarify the three-dimensional migration profile of interneurons derived from each part of the GE, we developed a technique involving focal electroporation into a small, defined portion of the telencephalic hemisphere. While the medial GE cells migrated laterally and spread widely throughout the cortex, the majority of the CGE cells migrated caudally toward the caudal-most end of the telencephalon. Time-lapse imaging and an in vivo immunohistochemical study confirmed the existence of a migratory stream depicted by a population of CGE cells directed caudally that eventually reached the hippocampus. Transplantation experiments suggested that the caudal direction of migration of the CGE cells was intrinsically determined as early as embryonic day 13.5. The caudal migratory stream is a novel migratory path for a population of CGE-derived interneurons passing from the subpallium to the hippocampus.
源自神经节隆起（GE），尤其是其尾侧部分（CGE）的中间神经元的迁移路径基本上仍不清楚。为了阐明源自GE各部分的中间神经元的三维迁移概况，我们开发了一种技术，即将局部电穿孔应用于端脑半球的一个小的、特定的部分。虽然内侧GE细胞向外侧迁移并广泛扩散到整个皮质，但大多数CGE细胞向尾侧迁移，朝向端脑的最尾端。延时成像和一项体内免疫组织化学研究证实了存在一条迁移流，由一群向尾侧定向的CGE细胞描绘，最终到达海马体。移植实验表明，CGE细胞向尾侧迁移的方向早在胚胎第13.5天就内在地确定了。尾侧迁移流是一群源自CGE的中间神经元从皮质下区通向海马体的一条新的迁移路径。
