De Campos Marina Pereira, Cechinel Filho Valdir, Da Silva Rosi Zanoni, Yunes Rosendo Augusto, Zacchino Susana, Juarez Sabina, Bella Cruz Rosana Cé, Bella Cruz Alexandre
Programa de Mestrado em Ciências Farmacêuticas/CCS and Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), Universidade do Vale do Itajaí (UNIVALI), 88302-202, Itajaí-SC, Brazil.
Biol Pharm Bull. 2005 Aug;28(8):1527-30. doi: 10.1248/bpb.28.1527.
We have studied the crude methanolic extract (CME), some fractions (hexane, dichloromethane and ethyl acetate) and four pure compounds: eupomatenoid-3 (1), eupomatenoid-5 (2), conocarpan (3) and orientin (4), from Piper solmsianum, for possible antifungal activity against 12 pathogenic fungi. The minimal inhibitory concentration (MIC) was determined and the experiments showed that the CME exhibited antifungal action against all the dermatophytes tested, with MIC values of between 20 microg/ml to 60 microg/ml. Similar activity also was verified for the hexane, dichloromethane and ethyl acetate fractions. However, the starting material (CME), and all the fractions, did not exert inhibitory effect against hyaline hyphomycetes and were only discretely active against the zigomycetes and yeasts. Compounds 2, 3 and 4 also exhibited pronounced activity against all the dermatophytes tested (MIC< or =1 to 9 microg/ml) with potency as high as the standard antifungal drug (ketoconazole). Compound 3 also exhibited activity against all the yeasts tested. In conclusion, the antifungal activity of P. solmsianum seems to be related mainly to the presence of compounds 2, 3 (neolignans) and 4 (flavonoid), however it was verified that another active compound, as yet unidentified, exists in the plant.
我们研究了来自索尔姆斯氏胡椒（Piper solmsianum）的粗甲醇提取物（CME）、一些馏分（己烷、二氯甲烷和乙酸乙酯）以及四种纯化合物：优波马亭-3（1）、优波马亭-5（2）、柯诺卡潘（3）和荭草苷（4）对12种致病真菌的潜在抗真菌活性。测定了最低抑菌浓度（MIC），实验表明CME对所有测试的皮肤癣菌均表现出抗真菌作用，MIC值在20微克/毫升至60微克/毫升之间。己烷、二氯甲烷和乙酸乙酯馏分也证实有类似活性。然而，起始原料（CME）以及所有馏分对透明丝孢菌均未产生抑制作用，对接合菌和酵母仅具有微弱活性。化合物2、3和4对所有测试的皮肤癣菌也表现出显著活性（MIC≤1至9微克/毫升），效力与标准抗真菌药物（酮康唑）相当。化合物3对所有测试的酵母也表现出活性。总之，索尔姆斯氏胡椒的抗真菌活性似乎主要与化合物2、3（新木脂素）和4（类黄酮）的存在有关，不过已证实该植物中存在另一种尚未鉴定的活性化合物。
