Miyoshi Sosuke, Flexman Jennifer A, Cross Donna J, Maravilla Kenneth R, Kim Yongmin, Anzai Yoshimi, Oshima Junko, Minoshima Satoshi
Department of Bioengineering, University of Washington, Seattle, USA.
Mol Imaging Biol. 2005 Jul-Aug;7(4):286-95. doi: 10.1007/s11307-005-0008-1.
Magnetic resonance imaging (MRI) can track labeled cells in the brain. The use of hemagglutinating virus of Japan envelopes (HVJ-Es) to effectively introduce the contrast agent to neural progenitor cells (NPCs) is limited to date despite their high NPC affinity.
HVJ-Es and Lipofectamine 2000 were compared as transfection vehicles of superparamagnetic iron oxide (SPIO). Labeled NPCs were examined for iron content, MRI signal change, and fundamental cell characteristics. Prussian Blue staining was used after differentiation to determine SPIO localization.
HVJ-Es transfected up to 12.5 +/- 8.8 times more SPIO into NPCs. HVJ-Es do not affect cell viability or differentiation capability. Superparamagnetic iron oxide was disseminated in both the soma and neurites.
These findings indicate that HVJ-Es are an effective vehicle for SPIO transfection of NPCs. The intracellular localization after differentiation raises the question as to the capability of MRI to distinguish cell migration from axonal or dendritic growth in vivo.
磁共振成像（MRI）能够追踪大脑中标记的细胞。尽管日本血凝病毒包膜（HVJ-Es）对神经祖细胞（NPCs）具有较高的亲和力，但迄今为止，利用其将造影剂有效导入NPCs的应用仍受到限制。
比较了HVJ-Es和脂质体2000作为超顺磁性氧化铁（SPIO）转染载体的效果。检测标记的NPCs的铁含量、MRI信号变化及基本细胞特性。分化后采用普鲁士蓝染色确定SPIO的定位。
HVJ-Es将多达12.5±8.8倍的SPIO转染到NPCs中。HVJ-Es不影响细胞活力或分化能力。超顺磁性氧化铁在胞体和神经突中均有分布。
这些发现表明，HVJ-Es是一种将SPIO转染到NPCs中的有效载体。分化后的细胞内定位引发了关于MRI在体内区分细胞迁移与轴突或树突生长能力的问题。
