Sato Itaru, Suzuki Tadahiko, Kobayashi Haruo, Tsuda Shuji
Laboratories of Veterinary Public Health, Faculty of Agriculture, Iwate University, Japan.
J Vet Med Sci. 2005 Jul;67(7):731-4. doi: 10.1292/jvms.67.731.
Japanese horse chestnut seed extract (HCSE) dose-dependently inhibited the autooxidation of linoleic acid (IC(50): 0.2 mg/ml), and the inhibition was almost complete at a concentration of 1 mg/ml. The HCSE scavenged DPPH (1,1-diphenyl-2-picrylhydrazyl) radicals and superoxide anions with EC(50)s of 0.65 and 0.21 mg/ml, respectively. However, it had no effect on hydrogen peroxide. The HCSE inhibited the genotoxicities of furylfuramide, N-methyl-N-nitrosourea, methyl methanesulfonate, mitomycin C, 2-aminoanthracene and aflatoxin B1 at a concentration of 1 mg/ml or more. Total polyphenol content of the HCSE was 21 mg/g (13 mg/g-seeds). These results indicate that the Japanese horse chestnut seed is an antioxidative and antimutagenic botanical resource.
日本七叶树种子提取物（HCSE）对亚油酸的自氧化具有剂量依赖性抑制作用（IC50：0.2毫克/毫升），在浓度为1毫克/毫升时抑制作用几乎完全。HCSE清除DPPH（1,1 - 二苯基 - 2 - 苦基肼）自由基和超氧阴离子的EC50分别为0.65和0.21毫克/毫升。然而，它对过氧化氢没有影响。HCSE在浓度为1毫克/毫升或更高时抑制了呋喃糠酰胺、N - 甲基 - N - 亚硝基脲、甲基磺酸甲酯、丝裂霉素C、2 - 氨基蒽和黄曲霉毒素B1的遗传毒性。HCSE的总多酚含量为21毫克/克（13毫克/克种子）。这些结果表明日本七叶树种子是一种具有抗氧化和抗诱变作用的植物资源。
