Sato Itaru, Kofujita Hisayoshi, Tsuda Shuji
Laboratory of Veterinary Public Health, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka 020-8550, Japan.
J Vet Med Sci. 2007 Jul;69(7):709-12. doi: 10.1292/jvms.69.709.
Japanese horse chestnut (Aesculus turbinata) seed extract inhibits the activity of cyclooxygenase (COX), but its active constituents have not been identified. In the present study, COX inhibitors were isolated from the hexane extract of this seed by means of 4 steps of liquid chromatography and were identified by gas chromatography/mass spectrometry and nuclear magnetic resonance. The COX inhibitors in the extract of Japanese horse chestnut seeds were identified as linoleic acid, linolenic acid, and oleic acid. Their efficacies were in the following order: linolenic acid = linoleic acid > oleic acid. These active constituents are C18 unsaturated fatty acids; stearic acid, a C18 saturated fatty acid, had no activity. Linolenic acid and linoleic acid had high selectivity toward COX-2 (selectivity index = 10), whereas oleic acid had no selectivity. Considering the efficacy and yield of each fatty acid, linoleic acid may be the principal COX inhibitor in this seed.
日本七叶树（Aesculus turbinata）种子提取物可抑制环氧化酶（COX）的活性，但其活性成分尚未确定。在本研究中，通过4步液相色谱法从该种子的己烷提取物中分离出COX抑制剂，并通过气相色谱/质谱和核磁共振进行鉴定。日本七叶树种子提取物中的COX抑制剂被鉴定为亚油酸、亚麻酸和油酸。它们的效力顺序如下：亚麻酸=亚油酸>油酸。这些活性成分是C18不饱和脂肪酸；硬脂酸，一种C18饱和脂肪酸，没有活性。亚麻酸和亚油酸对COX-2具有高选择性（选择性指数=10），而油酸没有选择性。考虑到每种脂肪酸的效力和产量，亚油酸可能是该种子中的主要COX抑制剂。
