人肿瘤细胞系条件培养基诱导人外周血淋巴细胞集落
The induction of human peripheral blood lymphoid colonies by conditioned media from human tumour cell lines.
作者信息
Vesole D H, Moore G E
出版信息
Immunology. 1980 Nov;41(3):723-8.
Conditioned medium (CM) from 29 human tumour cell lines and 3 malignant pleural fluids were tested for their ability to stimulate lymphoid colony formation in semi-solid agar; 9 of 14 malignant melanomas, 3 of 6 colonic carcinomas, 2 of 5 ovarian carcinomas, 3 of 4 breast carcinomas and 1 of 3 pleural fluids from breast cancer patients contained colony-stimulating activity (CSA) for human peripheral blood lymphoid cells (PBL) in semi-solid agar. Conditioned media also stimulated PBL proliferation in liquid medium; these effects were dose dependent. With the exception of one pleural fluid, extensive dialysis of CM did not significantly increase colony formation; CM from two tumour cell lines demonstrated a significant decrease in the induction of colony formation after dialysis.
检测了来自29种人类肿瘤细胞系的条件培养基（CM）和3份恶性胸腔积液刺激半固体琼脂中淋巴样集落形成的能力；14例恶性黑色素瘤中有9例、6例结肠癌中有3例、5例卵巢癌中有2例、4例乳腺癌中有3例以及3例乳腺癌患者的胸腔积液中有1例在半固体琼脂中含有对人外周血淋巴细胞（PBL）的集落刺激活性（CSA）。条件培养基还能刺激液体培养基中PBL的增殖；这些效应呈剂量依赖性。除一份胸腔积液外，CM的广泛透析并未显著增加集落形成；来自两个肿瘤细胞系的CM在透析后集落形成诱导显著降低。
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