对大肠杆菌aroA基因进行扩增可使其对除草剂草甘膦产生耐受性。
Amplification of the aroA gene from Escherichia coli results in tolerance to the herbicide glyphosate.
作者信息
Rogers S G, Brand L A, Holder S B, Sharps E S, Brackin M J
出版信息
Appl Environ Microbiol. 1983 Jul;46(1):37-43. doi: 10.1128/aem.46.1.37-43.1983.
Abstract
The predominant cellular target of the herbicide glyphosate is thought to be the enzyme 5-enolpyruvylshikimate-3-phosphoric acid synthase (EPSP synthase). As a means of biologically testing this finding, we cloned a segment of DNA from Escherichia coli that encodes this enzyme. Clones carrying the gene for EPSP synthase were identified by genetic complementation. Cells that contain a multicopy plasmid carrying the EPSP synthase gene overproduce the enzyme 5- to 17-fold and exhibit at least an 8-fold increased tolerance to glyphosate. These experiments provide direct biological evidence that EPSP synthase is a major site of glyphosate action in E. coli and that, in an amplified form, it can serve as a selectable glyphosate resistance marker.
摘要
除草剂草甘膦的主要细胞靶点被认为是5-烯醇丙酮酰莽草酸-3-磷酸合酶（EPSP合酶）。作为对这一发现进行生物学测试的一种方法，我们从大肠杆菌中克隆了一段编码该酶的DNA。通过遗传互补鉴定出携带EPSP合酶基因的克隆。含有携带EPSP合酶基因的多拷贝质粒的细胞，该酶的产量会过量产生5至17倍，并且对草甘膦的耐受性至少提高8倍。这些实验提供了直接的生物学证据，证明EPSP合酶是草甘膦在大肠杆菌中的主要作用位点，并且以扩增形式存在时，它可以作为一种可选择的草甘膦抗性标记。
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