Szabó K, Lichtenthaler H K, Kocsányi L, Richter P
Botanisches Institut II, Universität Karlsruhe, Federal Republic of Germany.
Radiat Environ Biophys. 1992;31(2):153-60. doi: 10.1007/BF01211213.
A new device for the measurement of complete laser induced fluorescence emission spectra (maxima near 690 and 735 nm) of leaves during the induction of the chlorophyll fluorescence is described. In this the excitation light (cw He/Ne laser, 632.8 nm) is switched on by a fast electro-mechanical shutter which provides an opening time of 1 ms. The emitted fluorescence is imaged onto the entrance slit of a multichannel spectrograph through a red cut-off filter (greater than 645 nm). A charge coupled device (CCD) sensor with 2048 elements simultaneously detects the complete chlorophyll fluorescence emission spectrum in the 650-800 nm wavelength range. Scanning is accomplished electronically and the integration time for a complete fluorescence emission spectrum can be selected from 10 ms up to 260 ms. Shutter, detector system and data acquisition are controlled by an IBM-PC/AT compatible computer. A maximum of 32 spectra can be measured at selected times during the fluorescence induction kinetics with the shortest time resolution of 10 ms. The instrument permits the determination of various fluorescence parameters: a) the rise-time of the fluorescence to the maximum level fm, b) the changes in the shape of the fluorescence emission spectra during the induction kinetics, c) the induction kinetics in the fluorescence ratio F690/F735 as well as d) the fluorescence decrease ratio Rfd at any wavelength between 650 to 800 nm. These fluorescence parameters provide information about the functioning of photosynthesis. The ratio F690/F735 allows the non-destructive determination of the chlorophyll content of leaves. The application of this instrument in ecophysiological research and stress physiology of plants is outlined.
描述了一种用于测量叶绿素荧光诱导过程中叶片完整激光诱导荧光发射光谱（最大值接近690和735 nm）的新装置。在此装置中，激发光（连续波氦氖激光器，632.8 nm）由快速机电快门开启，快门的开启时间为1 ms。发射的荧光通过一个红色截止滤光片（大于645 nm）成像到多通道光谱仪的入口狭缝上。一个具有2048个元件的电荷耦合器件（CCD）传感器同时检测650 - 800 nm波长范围内的完整叶绿素荧光发射光谱。扫描通过电子方式完成，完整荧光发射光谱的积分时间可在10 ms至260 ms之间选择。快门、探测器系统和数据采集由一台IBM - PC/AT兼容计算机控制。在荧光诱导动力学过程中的选定时间，最多可测量32个光谱，最短时间分辨率为10 ms。该仪器可测定各种荧光参数：a）荧光上升至最大水平fm的上升时间，b）诱导动力学过程中荧光发射光谱形状的变化，c）荧光比率F690/F735的诱导动力学，以及d）650至800 nm之间任何波长处的荧光下降比率Rfd。这些荧光参数提供了有关光合作用功能的信息。比率F690/F735可用于无损测定叶片的叶绿素含量。概述了该仪器在植物生态生理学研究和胁迫生理学中的应用。
