一种用于生物样品中多胺测定的改进且简便的技术。应用于肥大大鼠心脏的无细胞系统。

An improved and easy technique for polyamine determination in biological samples. Application to cell-free system from hypertrophied rat heart.

作者信息

Kneip B, Raymondjean M, Bogdanovsky D, Bachner L, Shapira G

出版信息

J Chromatogr. 1979 Apr 11;162(4):547-59. doi: 10.1016/s0378-4347(00)81834-4.

Abstract

An accurate, improved cation-exchange chromatographic method using o-phthalaldehyde and ultraviolet detection at 280 nm for the determination of free polyamines (putrescine, spermidine, spermine) has been developed. Different samples, such as the 105,000 g supernatant of reticulocyte or heart muscle, and KCl ribosomal wash containing initiation factors, can be analysed. The minor modification of reagents results in a good precision and sensitivity, which is demonstrated by a relative standard deviation of 5--9% and recoveries of 98%. This technique is of particular interest because it allows polyamine determination in biological samples with high concentrations of salt.

摘要

已开发出一种准确、改进的阳离子交换色谱法，该方法使用邻苯二甲醛并在280nm处进行紫外检测来测定游离多胺（腐胺、亚精胺、精胺）。可以分析不同的样品，如网织红细胞或心肌的105,000g上清液，以及含有起始因子的KCl核糖体洗涤液。试剂的微小改动带来了良好的精密度和灵敏度，相对标准偏差为5%-9%，回收率为98%，证明了这一点。这项技术特别引人关注，因为它能够测定高盐浓度生物样品中的多胺。