Tadolini B, Cabrini L, Piccinini G, Davalli P P, Sechi A M
Appl Biochem Biotechnol. 1985 Jun;11(3):173-6. doi: 10.1007/BF02798473.
Heparin-sepharose has been utilized to remove polyamines adsorbed to the cytoplasmic surface of rat heart mitochondria. The results obtained can be summarized as follows: Heparin-sepharose removes 90% of the spermine, 98% of the spermidine, and 98% of the putrescine adsorbed. Polyamine contents of chromatographed mitochondria amount to 2.66 and 0.36 nmol spermine and spermidine, respectively, per mg of mitochondrial protein.
肝素琼脂糖已被用于去除吸附在大鼠心脏线粒体细胞质表面的多胺。所得结果可总结如下：肝素琼脂糖可去除90%的精胺、98%的亚精胺和98%的腐胺。经色谱分析的线粒体中多胺含量分别为每毫克线粒体蛋白2.66纳摩尔精胺和0.36纳摩尔亚精胺。
