Pekkinen M, Varvio S, Kulju K K M, Kärkkäinen H, Smolander S, Viherä-Aarnio A, Koski V, Sillanpää M J
Department of Applied Chemistry and Microbiology, University of Helsinki, Finland.
Genome. 2005 Aug;48(4):619-25. doi: 10.1139/g05-031.
The first genetic linkage map for silver birch, Betula pendula Roth, was constructed by using a pseudo-testcross mapping strategy and integration of linkage data from 3 unrelated 2-generation pedigrees. The map is based on the genetic inheritance and segregation of 82 amplified fragment length polyhmorphisms and 19 microsatellite markers, and was constructed by simultaneously comparing the performance of CRI-MAP and OUTMAP packages. The analysis revealed 16 linkage groups, and the total map coverage is 1561 cM (Kosambi units). Average map distance between adjacent markers is 15.5 cM. Linkage groups range between 6 and 18 loci and from 81.2 to 326.5 cM; the remaining 9 linkage groups consist of 2 or 3 loci ranging from 6.3 to 42.4 cM. The uncertainty of the map is illustrated with sensitivity analysis. This initial map can serve as a basis for developing a more detailed genetic map.
利用拟测交作图策略并整合来自3个不相关的二代谱系的连锁数据，构建了银桦（Betula pendula Roth）的首张遗传连锁图谱。该图谱基于82个扩增片段长度多态性和19个微卫星标记的遗传传递和分离情况，并通过同时比较CRI-MAP和OUTMAP软件包的性能构建而成。分析揭示了16个连锁群，图谱总覆盖度为1561厘摩（科斯ambi单位）。相邻标记间的平均图谱距离为15.5厘摩。连锁群包含6至18个位点，长度在81.2至326.5厘摩之间；其余9个连锁群由2或3个位点组成，长度在6.3至42.4厘摩之间。通过敏感性分析说明了图谱的不确定性。这一初步图谱可作为构建更详细遗传图谱的基础。
