Barkley N A, Newman M L, Wang M L, Hotchkiss M W, Pederson G A
USDA-ARS, Plant Genetic Resources Conservation Unit, Griffin, GA 30223, USA.
Genome. 2005 Aug;48(4):731-7. doi: 10.1139/g05-022.
Polymorphic expressed sequence tag - simple sequence repeat (EST-SSR) markers derived from major cereal crops were used to assess the genetic diversity of the USDA temperate bamboo collection consisting of 92 accessions classified in 11 separate genera and 44 species. A total of 211 bands were detected with a mean number of alleles per locus of 8.440. Phylogenetic relationships were determined by calculating genetic distances between all pairwise combinations and assessing differences in character data. The resulting dendrograms (unweighted pair group method with arithmetic means (UPGMA) and parsimony) clustered the accessions into 2 main clades, which corresponded to accessions characterized morphologically as either clumping (sympodial) or running (monopodial) bamboos. The majority of the accessions clustered according to their current taxonomic classification. These markers were also beneficial in identifying contaminated and (or) misidentified plots. Overall, these transferred markers were informative in differentiating the various bamboo accessions and determining the level of genetic variation within and among species and genera.
利用源自主要谷类作物的多态性表达序列标签-简单序列重复（EST-SSR）标记，对美国农业部温带竹子种质资源库的遗传多样性进行评估。该种质资源库包含92份种质，分属于11个不同属的44个物种。共检测到211条带，每个位点的平均等位基因数为8.440。通过计算所有成对组合之间的遗传距离并评估性状数据差异，确定系统发育关系。所得的聚类图（算术平均数的非加权配对组法（UPGMA）和简约法）将种质聚为2个主要分支，这与形态学上归类为丛生（合轴型）或散生（单轴型）竹子的种质相对应。大多数种质根据其当前的分类学分类进行聚类。这些标记对于识别受污染和（或）错误鉴定的样本也很有帮助。总体而言，这些转移标记在区分不同竹子种质以及确定种内和属内及属间的遗传变异水平方面具有信息价值。
