Regional Plant Resource Centre, Nayapalli, Bhubaneswar, Orissa, India.
Biochem Genet. 2010 Dec;48(11-12):1015-25. doi: 10.1007/s10528-010-9390-8. Epub 2010 Oct 26.
Genetic relationships among 22 taxa of bamboo were evaluated using 12 inter simple sequence repeats (ISSR) and four expressed sequence tag (EST)-based random primers, resulting in amplification of 220 loci. The grouping of species based on Jaccard's similarity matrix using UPGMA and principal coordinate analysis agreed with earlier published reports on molecular phylogenetic studies in bamboos with few deviations. In the dendrogram, however, species of one genus were placed in different clusters along with members of other genera. This calls for correct taxonomic delineation at the genus and species level using both vegetative and reproductive characters and correlation of molecular data with morphologically definable taxonomic groupings at the proper taxonomic level.
利用 12 对 ISSR 和 4 对 EST 随机引物，对 22 个竹类种进行了遗传关系评估，共扩增出 220 个位点。基于 Jaccard 相似性矩阵的 UPGMA 和主坐标分析对物种进行分组，与竹子分子系统发育研究的早期发表报告基本一致，仅有少数差异。然而，在聚类图中，一个属的物种与其他属的成员一起被放置在不同的聚类中。这需要使用营养和生殖特征，以及分子数据与适当分类水平的形态可定义分类群的相关性，对属和种进行正确的分类学界定。
