Johnson Cotteka N, Adkins Nicholas L, Georgel Philippe
Division of Biological Sciences, Marshall University, Huntington, WV 25755, USA.
Biochem Cell Biol. 2005 Aug;83(4):405-17. doi: 10.1139/o05-115.
Since the initial characterization of chromatin remodeling as an ATP-dependent process, many studies have given us insight into how nucleosome-remodeling complexes can affect various nuclear functions. However, the multistep DNA-histone remodeling process has not been completely elucidated. Although new studies are published on a nearly weekly basis, the nature and roles of interactions of the individual SWI/SNF- and ISWI-based remodeling complexes and DNA, core histones, and other chromatin-associated proteins are not fully understood. In addition, the potential changes associated with ATP recruitment and its subsequent hydrolysis have not been fully characterized. This review explores possible mechanisms by which chromatin-remodeling complexes are recruited to specific loci, use ATP hydrolysis to achieve actual remodeling through disruption of DNA-histone interactions, and are released from their chromatin template. We propose possible roles for ATP hydrolysis in a chromatin-release/target-scanning process that offer an alternative to or complement the often overlooked function of delivering the energy required for sliding or dislodging specific subsets of core histones.
自从染色质重塑最初被描述为一个依赖ATP的过程以来，许多研究让我们深入了解了核小体重塑复合物如何影响各种核功能。然而，多步骤的DNA-组蛋白重塑过程尚未完全阐明。尽管几乎每周都有新的研究发表，但基于SWI/SNF和ISWI的单个重塑复合物与DNA、核心组蛋白及其他染色质相关蛋白之间相互作用的性质和作用仍未完全了解。此外，与ATP募集及其随后水解相关的潜在变化也尚未得到充分表征。本综述探讨了染色质重塑复合物被募集到特定位点的可能机制，利用ATP水解通过破坏DNA-组蛋白相互作用来实现实际重塑，并从其染色质模板上释放的过程。我们提出了ATP水解在染色质释放/靶点扫描过程中的可能作用，这为滑动或去除核心组蛋白特定亚群所需能量传递这一常被忽视的功能提供了一种替代或补充。
