Cox N A, Bailey J S, Richardson L J, Buhr R J, Cosby D E, Wilson J L, Hiett K L, Siragusa G R, Bourassa D V
USDA-ARS, PMSRU, Russell Research Center, 950 College Station Road, Athens, GA 30605, USA.
Avian Dis. 2005 Jun;49(2):285-7. doi: 10.1637/7324-011005.
Campylobacter and Salmonella are known to cause acute bacterial gastroenteritis in humans. Raw poultry products have been implicated as a significant source of these infections. Five trials were conducted to determine whether Campylobacter and Salmonella spp. exist naturally in the mature and immature ovarian follicles of late-life broiler breeder hens. Broiler breeder hens ranging from 60 to 66 wk of age were obtained from four different commercial breeder operations. For each trial, the hens were removed from the commercial operation and held overnight at the University of Georgia processing facility. The hens were euthanized, defeathered, and aseptically opened. To reduce the possibility of cross-contamination between samples, first the mature and immature ovarian follicles, then the ceca, were aseptically removed. Individual samples were placed in sterile bags, packed on ice, and transported to the laboratory for evaluation. Overall, Campylobacter was found in 7 of 55 immature follicles, 12 of 47 mature follicles, and 41 of 55 ceca. Campylobacter was found in at least one of each sample of mature follicles and in ceca in each of the five trials. Salmonella was found in 0 of 55 immature follicles, 1 of 47 mature follicles, and 8 of 55 ceca. In this study, the recovery rate of Salmonella from late-life broiler breeder hen ovarian follicles was relatively low. However, the recovery rate of Campylobacter from the hen ovarian follicles was reasonably high, suggesting that these breeder hens could be infecting fertile hatching eggs. Determining how Campylobacter contaminated these ovarian follicles and how many chicks could be colonized from this source are the next steps in helping to elucidate a better understanding of this ecology and the control of Campylobacter in poultry production.
已知弯曲杆菌和沙门氏菌可导致人类急性细菌性肠胃炎。生禽产品被认为是这些感染的重要来源。开展了五项试验，以确定弯曲杆菌和沙门氏菌属是否自然存在于老龄肉种鸡的成熟和未成熟卵巢卵泡中。从四个不同的商业种鸡养殖场获取了60至66周龄的肉种鸡。每次试验时，将母鸡从商业养殖场取出，并在佐治亚大学加工设施中过夜饲养。对母鸡实施安乐死、拔毛并进行无菌剖检。为降低样本间交叉污染的可能性，先无菌取出成熟和未成熟卵巢卵泡，然后取出盲肠。将单个样本放入无菌袋中，用冰包装，运至实验室进行评估。总体而言，在55个未成熟卵泡中的7个、47个成熟卵泡中的12个以及55个盲肠中的41个中发现了弯曲杆菌。在五项试验的每项试验中，在每个成熟卵泡样本以及盲肠样本中至少发现了一个弯曲杆菌。在55个未成熟卵泡中的0个、47个成熟卵泡中的1个以及55个盲肠中的8个中发现了沙门氏菌。在本研究中，从老龄肉种鸡卵巢卵泡中检出沙门氏菌的比率相对较低。然而，从母鸡卵巢卵泡中检出弯曲杆菌的比率相当高，这表明这些种鸡可能会感染可孵化的种蛋。确定弯曲杆菌如何污染这些卵巢卵泡以及有多少雏鸡会由此来源定植，是进一步更好地了解这种生态以及控制家禽生产中弯曲杆菌的后续步骤。
