Lim Bum-Soon, Lee Shin-Jae, Lee Jae-Won, Ahn Sug-Joon
Dental Research Institute and Department of Dental Biomaterials Science, College of Dentistry, Seoul National University, Seoul, Korea, ROK.
Am J Orthod Dentofacial Orthop. 2008 Jun;133(6):882-8. doi: 10.1016/j.ajodo.2006.07.027.
Knowledge of adhesion patterns of cariogenic streptococci to orthodontic materials can provide valuable information on the cause of enamel demineralization during orthodontic treatment. The purpose of this study was to investigate the adhesion of 2 cariogenic streptococci strains to 7 orthodontic raw materials (3 light-cured orthodontic adhesives, 3 bracket raw materials, and hydroxyapatite) with respect to bacterial species, incubation time, and saliva coating.
Each material was incubated with unstimulated whole saliva or phosphate-buffered saline solution for 2 hours. Binding assays were then performed by incubating tritium-labeled cariogenic streptococci with each raw material for 3 or 6 hours.
The degree of adhesion varied by material type. Generally, adhesion of cariogenic streptococci was significantly higher for bonding adhesives than for bracket materials, and adhesion to resin-modified glass ionomer was the highest. A longer incubation time generally increased bacterial adhesion, whereas saliva coating did not significantly influence bacterial adhesion.
Bonding adhesives around brackets should be removed carefully during the bonding procedure to avoid enamel decalcification.
了解致龋性链球菌与正畸材料的黏附模式可为正畸治疗期间牙釉质脱矿的原因提供有价值的信息。本研究的目的是就细菌种类、孵育时间和唾液包被情况，研究2种致龋性链球菌菌株对7种正畸原材料（3种光固化正畸黏结剂、3种托槽原材料和羟基磷灰石）的黏附情况。
每种材料与未刺激的全唾液或磷酸盐缓冲盐溶液孵育2小时。然后通过将氚标记的致龋性链球菌与每种原材料孵育3或6小时进行结合试验。
黏附程度因材料类型而异。一般来说，致龋性链球菌在黏结剂上的黏附显著高于托槽材料，且对树脂改性玻璃离子体的黏附最高。较长的孵育时间通常会增加细菌黏附，而唾液包被对细菌黏附没有显著影响。
在黏结过程中应小心去除托槽周围的黏结剂，以避免牙釉质脱钙。
