Sadeghi M R, Lakpour N, Heidari-Vala H, Hodjat Mahshid, Amirjannati N, Hossaini Jadda H, Binaafar Sima, Akhondi M M
Reproductive Biotechnology Research Center, Avicenna Research Institute, Tehran, Iran.
Rom J Morphol Embryol. 2011;52(2):645-51.
This study was done to evaluate the effect of sperm source on chromatin integrity and ICSI outcomes. One hundred and thirteen samples containing epididymal aspirates of 57 obstructive azoospermic men and 56-ejaculated semen of normozoospermic men were included in this study. Sperm chromatin status was evaluated by Chromomycin A3 (CMA3), Aniline Blue (AB) and Toluidine Blue (TB). Fertilization rate and embryo quality were recorded. In epididymal group the percentage of sperms stained with AB, CMA3 and TB were significantly higher compared to ejaculate group while fertilization rate (60.6% vs. 74.04%) was significantly lower. However, embryo quality was not significantly different between two groups. In addition, abnormal sperm chromatin condensation and DNA fragmentation were not correlated with fertilization rate and embryo quality. Our results highlight the role of epididymis in sperm maturation and confirm that ICSI using ejaculated sperm is the gold standard for treatment of infertile men.
本研究旨在评估精子来源对染色质完整性和卵胞浆内单精子注射（ICSI）结局的影响。本研究纳入了113份样本，其中包括57名梗阻性无精子症男性的附睾抽吸物和56名正常精子症男性的射出精液。通过吖啶橙（CMA3）、苯胺蓝（AB）和甲苯胺蓝（TB）评估精子染色质状态。记录受精率和胚胎质量。附睾组中，AB、CMA3和TB染色的精子百分比显著高于射出精液组，而受精率（60.6%对74.04%）显著较低。然而，两组间胚胎质量无显著差异。此外，异常精子染色质浓缩和DNA碎片与受精率及胚胎质量无关。我们的结果突出了附睾在精子成熟中的作用，并证实使用射出精子进行ICSI是治疗不育男性的金标准。
