Hammadeh M E, Strehler E, Zeginiadou T, Rosenbaum P, Schmidt W
Department of Obstetrics and Gynecology, University of Saarland, Homburg, Germany.
Arch Androl. 2001 Apr-Jun;47(2):83-7. doi: 10.1080/014850101316901262.
The inability of sperm chromatin to decondense has been implicated in the failure of fertilization, This study was undertaken to identify the relationship between sperm chromatin decondensation in vitro after incubation with follicular fluid at various points in time and fertilization or pregnancy rates after intracytoplasmic sperm injection. Moreover, an attempt was made to determine whether this test could be used as a predictive test for the outcome of ICSI. Thirty-two infertile couples undergoing ICSI therapy were included in this prospective study. One milliter of semen from each sample was mixed with 1 mL of follicular fluid obtained from ICSI patients at the time of oocyte retrieval and incubated for 24 h. Many smears were made directly after semen liquefaction at the following time intervals: 30, 60, and 120 min and 24 h. Chromatin decondensation was evaluated with acridine orange staining. The mean percentage of uncondensed chromatin of spermatozoa in the native semen samples was 25 +/- 18.3%, which increased within 24 h to 91 +/- 9.5%. On the other hand, the fertilization and ongoing pregnancy rates were 64 +/- 21.7% and 20%, respectively. However, no correlations were found between chromatin decondensation at various point of time (30, 60, and 120 min and 24 h) and fertilization rate. No correlation was shown between the chromatin decondensation and sperm counts in the ejaculate. morphology, or the percentage of condensed chromatin. In light of this study, chromatin decondensation in vitro cannot be recommended for predicting the fertilization potential of spermatozoa and pregnancy rates in the ICSI program. Further research is necessary, especially in cases where ICSI is being considered as a therapeutic option.
精子染色质无法解聚被认为与受精失败有关。本研究旨在确定在不同时间点与卵泡液孵育后精子染色质体外解聚与卵胞浆内单精子注射后的受精率或妊娠率之间的关系。此外，还试图确定该测试是否可作为卵胞浆内单精子注射结果的预测性测试。这项前瞻性研究纳入了32对接受卵胞浆内单精子注射治疗的不育夫妇。从每个样本中取1毫升精液与1毫升在取卵时从接受卵胞浆内单精子注射的患者获取的卵泡液混合，并孵育24小时。在精液液化后的以下时间间隔直接制作多个涂片：30、60和120分钟以及24小时。用吖啶橙染色评估染色质解聚情况。天然精液样本中精子未凝聚染色质的平均百分比为25±18.3%，在24小时内增加到91±9.5%。另一方面，受精率和持续妊娠率分别为64±21.7%和20%。然而，在不同时间点（30、60和120分钟以及24小时）的染色质解聚与受精率之间未发现相关性。染色质解聚与射精中的精子计数、形态或凝聚染色质的百分比之间也未显示相关性。根据本研究，不建议将体外染色质解聚用于预测卵胞浆内单精子注射程序中精子的受精潜力和妊娠率。有必要进行进一步研究，特别是在考虑将卵胞浆内单精子注射作为治疗选择的情况下。
