Teichmann Alexa, Jacobi Ute, Ossadnik Michael, Richter Heike, Koch Stefan, Sterry Wolfram, Lademann Jürgen
Center of Experimental and Applied Cutaneous Physiology, Department of Dermatology, Medical Faculty Charité, Berlin, Germany.
J Invest Dermatol. 2005 Aug;125(2):264-9. doi: 10.1111/j.0022-202X.2005.23779.x.
The determination of penetration pathways of topically applied substances into the skin is the subject of several investigations. Recently, follicular penetration has become a major focus of interest. To date, a direct, non-invasive quantification of the amount of topically applied substance penetrated into the follicles had not been possible. The development of such a method was the aim of this study. Therefore, the advantages of both stripping techniques, tape stripping and cyanoacrylate skin surface biopsy, were combined and evaluated. Tape stripping was used to remove the part of the stratum corneum that contained the topically applied dye. Subsequently, the follicular contents were ripped off by cyanoacrylate skin surface biopsy. The combined method termed "differential stripping" was evaluated in vitro and in vivo, and the amount of topically applied fluorescent dye penetrated into the hair follicles was quantified after different penetration times. After 30 min, 5% of the recovered concentration of sodium fluorescein was found in the follicular infundibula, where it was still detectable after 48 h. Altogether, the results of this investigation revealed that differential stripping is a new method that can be used to study the penetration of topically applied substances into the follicular infundibula non-invasively and selectively.
局部应用物质进入皮肤的渗透途径的确定是多项研究的主题。最近，毛囊渗透已成为主要的研究热点。迄今为止，还无法直接、无创地定量局部应用物质进入毛囊的量。本研究的目的就是开发这样一种方法。因此，将胶带剥离和氰基丙烯酸酯皮肤表面活检这两种剥离技术的优点结合起来并进行评估。使用胶带剥离去除含有局部应用染料的角质层部分。随后，通过氰基丙烯酸酯皮肤表面活检撕下毛囊内容物。这种称为“差异剥离”的联合方法在体外和体内进行了评估，并在不同渗透时间后对局部应用的荧光染料进入毛囊的量进行了定量。30分钟后，在毛囊漏斗部发现了5%回收浓度的荧光素钠，48小时后仍可检测到。总之，本研究结果表明，差异剥离是一种可用于无创、选择性地研究局部应用物质进入毛囊漏斗部渗透情况的新方法。
