Herken R, Eibs H G, Spielmann H
Bibl Anat. 1981(19):247-57.
To explain the teratogenic action of cyclophosphamide treatment during the preimplantation period (day 3; 60 mg/kg), the decidual reaction of the uterus was studied in the rat by light microscopy and autoradiography on day 4 (preimplantation period) and on days 5 and 6 (implantation period). On day 4, 24 h after cyclophosphamide treatment, the 3H-thymidine labelling index of the stroma cells beneath the uterine epithelium was significantly increased (33.7 +/- 5.2%) as compared to untreated control animals (6.5 +/- 0.4%). In addition, the surface of the uterine lumina appeared smoother in treated animals than in controls. On day 5 of gestation no difference could be detected autoradiographically between the uteri and treated and untreated animals. Histologically, however, the decidual reaction in treated animals occupied a larger area than in controls. On day 6 of pregnancy the 3H-labelling index of stroma cells surrounding the implantation site of treated animals was considerably reduced (10.7 vs. 32.8% in controls) and necrotic stroma cells were found in the area around the implantation site. Mechanisms inducing the decidual changes in cyclophosphamide-treated animals are discussed.
为解释着床前期（第3天；60毫克/千克）环磷酰胺治疗的致畸作用，通过光学显微镜和放射自显影技术，对大鼠在第4天（着床前期）以及第5天和第6天（着床期）的子宫蜕膜反应进行了研究。在第4天，即环磷酰胺治疗24小时后，与未治疗的对照动物（6.5±0.4%）相比，子宫上皮下方基质细胞的3H-胸腺嘧啶核苷标记指数显著升高（33.7±5.2%）。此外，治疗组动物子宫腔表面比对照组看起来更光滑。在妊娠第5天，通过放射自显影法未检测到治疗组和未治疗组动物子宫之间的差异。然而，从组织学上看，治疗组动物的蜕膜反应区域比对照组更大。在妊娠第6天，治疗组动物着床部位周围基质细胞的3H标记指数显著降低（10.7%，而对照组为32.8%），并且在着床部位周围区域发现了坏死的基质细胞。本文讨论了环磷酰胺治疗动物中诱导蜕膜变化的机制。
