水溶液中铽（III）-两种氟喹诺酮-亚硫酸钠体系的电化学发光

Electrochemiluminescence of terbium (III)-two fluoroquinolones-sodium sulfite system in aqueous solution.

作者信息

Chen Shi-lv, Ding Fen, Liu Yu, Zhao Hui-chun

机构信息

Department of Chemistry, Beijing Normal University, Beijing 100875, PR China.

出版信息

Spectrochim Acta A Mol Biomol Spectrosc. 2006 May 1;64(1):130-5. doi: 10.1016/j.saa.2005.07.006. Epub 2005 Aug 10.

DOI:10.1016/j.saa.2005.07.006

PMID:16098798

Abstract

The electrochemiluminescence (ECL) of Tb3+-enoxacin-Na2SO3 system (ENX system) and Tb3+-ofloxacin-Na2SO3 system (OFLX system) in aqueous solution is reported. ECL is generated by the oxidation of Na2SO3, which is enhanced by Tb3+-fluoroquinolone (FQ) complex. The ECL intensity peak versus potential corresponds to oxidation of Na2SO3, and the ECL emission spectra (the peaks are at 490, 545, 585 and 620 nm) match the characteristic emission spectrum of Tb3+, indicating that the emission is from the excited state of Tb3+. The mechanism of ECL is proposed and the difference of ECL intensity between ENX system and OFLX system is explained. Conditions for ECL emission were optimized. The linear range of ECL intensity versus concentrations of pharmaceuticals is 2.0 x 10(-10) -8.0 x 10(-7)mol l(-1) for ENX and 6.0 x 10(-10) -6.0 x 10(-7)mol l(-1) for OFLX, respectively. A theoretical limit of detection is 5.4 x 10(-11)mol l(-1) for ENX and 1.6 x 10(-10)mol l(-1) for OFLX, respectively. The ECL was satisfactorily applied to the determination of the two FQs in dosage form and urine sample.

摘要

报道了水溶液中Tb3+-依诺沙星-Na2SO3体系（ENX体系）和Tb3+-氧氟沙星-Na2SO3体系（OFLX体系）的电化学发光（ECL）。ECL由Na2SO3的氧化产生，Tb3+-氟喹诺酮（FQ）络合物可增强该氧化过程。ECL强度峰与电位对应于Na2SO3的氧化，且ECL发射光谱（峰位于490、545、585和620nm处）与Tb3+的特征发射光谱匹配，表明发射来自Tb3+的激发态。提出了ECL的机理并解释了ENX体系和OFLX体系之间ECL强度的差异。优化了ECL发射的条件。对于ENX，ECL强度与药物浓度的线性范围分别为2.0×10(-10)-8.0×10(-7)mol l(-1)，对于OFLX为6.0×10(-10)-6.0×10(-7)mol l(-1)。ENX的理论检测限分别为5.4×10(-11)mol l(-1)，OFLX为1.6×10(-10)mol l(-1)。该ECL方法成功应用于剂型和尿液样品中两种氟喹诺酮类药物的测定。