Department of Hepatobiliary Surgery, The Affiliated Xingtai People's Hospital of Hebei Medical University; Institute of Tumor Disease of Xingtai City, Xingtai, Hebei 054001, P.R. China.
Department of Medicine, General Hospital of Jizhong Energy Xingtai Mining Group Limited Liability Company, Xingtai, Hebei 054000, P.R. China.
Oncol Rep. 2017 Oct;38(4):2166-2172. doi: 10.3892/or.2017.5906. Epub 2017 Aug 14.
In recent years it was found that the synthesis and biological activity of ribosomes are closely associated with tumor cell growth, tumorigenesis, and malignant transformation. However, the role of regulator of ribosome synthesis 1 (RRS1) in hepatocellular carcinoma (HCC) has not yet been reported. In the present study, we aimed to examine the potential role of RRS1 in tumor cell growth by using a lentivirus-mediated RNA interference (RNAi) system in the HCC cell line SMMC-7721 in vitro. Compared with that of the negative control group (Lv-shCon), the mRNA and protein expression levels of RRS1 in SMMC-7721 cells transfected with Lv-shRRS1 were significantly decreased. Further experiments found that silencing of RRS1 gene expression in SMMC-7721 cells significantly suppressed cell proliferation, inhibited colony formation capacity, increased apoptosis and arrested cells in the G1 phase. These results suggest that the RRS1 gene plays a critical role in cell proliferation, colony formation, cell apoptosis and cell cycle distribution in human HCC cells, and that silencing of RRS1 by RNAi is a promising therapeutic approach for the treatment of HCC, and should be further developed.
近年来，人们发现核糖体的合成和生物活性与肿瘤细胞的生长、肿瘤发生和恶性转化密切相关。然而，核糖体合成调节因子 1（RRS1）在肝细胞癌（HCC）中的作用尚未报道。本研究旨在通过慢病毒介导的 RNA 干扰（RNAi）系统在 HCC 细胞系 SMMC-7721 中研究 RRS1 在肿瘤细胞生长中的潜在作用。与阴性对照组（Lv-shCon）相比，Lv-shRRS1 转染的 SMMC-7721 细胞中 RRS1 的 mRNA 和蛋白表达水平显著降低。进一步的实验发现，沉默 SMMC-7721 细胞中 RRS1 基因的表达显著抑制细胞增殖，抑制集落形成能力，增加细胞凋亡并将细胞阻滞在 G1 期。这些结果表明，RRS1 基因在人 HCC 细胞的增殖、集落形成、细胞凋亡和细胞周期分布中起关键作用，通过 RNAi 沉默 RRS1 是治疗 HCC 的一种有前途的治疗方法，应进一步开发。
