Department of Biochemistry, Leiden University, Einsteinweg 5, 2300 RA Leiden, The Netherlands.
Appl Environ Microbiol. 1990 Nov;56(11):3458-62. doi: 10.1128/aem.56.11.3458-3462.1990.
A new method for the quantification and characterization of manganese-oxidizing activity by spent culture medium of Leptothrix discophora SS-1 was developed. It is based on the formation of the dye Wurster blue from N,N,N',N'-Tetramethyl-p-phenylenediamine by oxidized manganese generated in the spent medium. The kinetic parameters thus obtained agreed well with data obtained with other methods. It was also possible to demonstrate iron oxidation by spent culture medium. The kinetics of the process and inhibition by enzyme poisons suggest that iron oxidation is enzymatically catalyzed. Probably two different factors are involved in manganese and iron oxidation.
开发了一种通过鞘铁细菌 SS-1 的废培养液定量和表征锰氧化活性的新方法。它基于在废培养液中生成的氧化锰使 N,N,N',N'-四甲基-p-苯二胺形成染料 Wurster 蓝。由此获得的动力学参数与用其他方法获得的数据吻合良好。也可以证明废培养液中铁的氧化。该过程的动力学和酶抑制剂的抑制作用表明，铁的氧化是酶促催化的。锰和铁氧化可能涉及两种不同的因素。
