Aizawa M, Yoshimaru H, Saito H, Katsuki T, Kawahara T, Kitamura K, Shi F, Kaji M
Graduate School of Frontier Sciences, The University of Tokyo, 5-1-5, Kashiwanoha, Kashiwa, Chiba 277-8563, Japan.
Mol Ecol. 2007 Aug;16(16):3393-405. doi: 10.1111/j.1365-294X.2007.03391.x.
Range-wide genetic variation of the widespread cold-temperate spruce Picea jezoensis was studied throughout northeast Asia using maternally inherited mitochondrial DNA and paternally inherited chloroplast DNA markers. This study assessed 33 natural populations including three varieties of the species in Japan, Russia, China, and South Korea. We depicted sharp suture zones in straits around Japan in the geographical distribution pattern of mitochondrial haplotypes (GST=0.901; NST=0.934). In contrast, we detected possible extensive pollen flow without seed flow across the straits around Japan during the past population history in the distribution pattern of chloroplast haplotypes (GST=0.233; NST=0.333). The analysis of isolation by distance of the species implied that by acting as a barrier for the movement of seeds and pollen, the sharp suture zones contributed considerably to the level of genetic differentiation between populations. Constructed networks of mitochondrial haplotypes allowed inference of the phylogeographical history of the species. We deduced that the disjunction with Kamchatka populations reflects range expansion and contraction to the north of the current distribution. Within Japan, we detected phylogeographically different types of P. jezoensis between Hokkaido and Honshu islands; P. jezoensis in Honshu Island may have colonized this region from the Asian continent via the Korean peninsula and the species in Hokkaido Island is likely to have spread from the Asian continent via Sakhalin through land bridges. Japanese endemism of mitochondrial haplotypes in Hokkaido and Honshu islands might have been promoted by separation of these islands from each other and from the Asian continent by the straits during the late Quaternary.
利用母系遗传的线粒体DNA和父系遗传的叶绿体DNA标记，在整个东北亚地区研究了广泛分布的寒温带云杉——鱼鳞云杉的全范围遗传变异。本研究评估了33个自然种群，包括日本、俄罗斯、中国和韩国该物种的三个变种。在线粒体单倍型的地理分布模式中，我们在日本周围的海峡中描绘出明显的缝合带（GST = 0.901；NST = 0.934）。相比之下，在叶绿体单倍型的分布模式中，我们在过去的种群历史中检测到可能存在的广泛花粉流，而种子流未穿过日本周围的海峡（GST = 0.233；NST = 0.333）。对该物种的距离隔离分析表明，这些明显的缝合带作为种子和花粉移动的屏障，对种群间的遗传分化水平有很大贡献。构建的线粒体单倍型网络有助于推断该物种的系统地理学历史。我们推断，与堪察加半岛种群的间断分布反映了当前分布范围向北的扩张和收缩。在日本境内，我们在北海道和本州岛之间检测到系统地理学上不同类型的鱼鳞云杉；本州岛的鱼鳞云杉可能是通过朝鲜半岛从亚洲大陆殖民到该地区的，而北海道岛的该物种很可能是通过萨哈林岛经陆桥从亚洲大陆传播而来的。北海道和本州岛线粒体单倍型的日本特有性可能是由于这些岛屿在第四纪晚期通过海峡彼此分离并与亚洲大陆分离而促进的。
