Matsunaga T, Nakasono S, Takamuku T, Burgess J G, Nakamura N, Sode K
Department of Biotechnology, Tokyo University of Agriculture and Technology, Japan.
Appl Environ Microbiol. 1992 Feb;58(2):686-9. doi: 10.1128/aem.58.2.686-689.1992.
A novel electrochemical reactor employing carbon-cloth electrodes was constructed for disinfection of drinking water. Escherichia coli K-12 (10(2) cells per cm3) was sterilized when a cell suspension was passed through the reactor at a dilution rate of 6.0 h-1, and a potential of 0.7 V versus a saturated calomel electrode was applied to an electrode. The survival ratio increased with increasing dilution rate but was less than 0.1% at dilution rates of less than 6.0 h-1. Although the survival ratio increased with increasing cell concentration above 10(3) cells per cm3, the disinfection rate also increased. The disinfection rate was 6.0 x 10(2) cells per cm3 per h at a cell concentration of 10(2) cells per cm3. Continuous sterilization of E. coli cells was carried out for 24 h. Sterilization is based on an electrochemical reaction between the electrode and the cell which is mediated by intracellular coenzyme A. Sterilization of drinking water by using this reactor was successfully performed, demonstrating the potential of such a reactor for clean and efficient water purification.
构建了一种采用碳布电极的新型电化学反应器用于饮用水消毒。当细胞悬浮液以6.0 h⁻¹的稀释率通过反应器，并向电极施加相对于饱和甘汞电极0.7 V的电位时，大肠杆菌K - 12（每立方厘米10²个细胞）被灭菌。存活率随稀释率增加而升高，但在稀释率低于6.0 h⁻¹时小于0.1%。尽管当细胞浓度高于每立方厘米10³个细胞时存活率随细胞浓度增加而升高，但消毒率也增加。在细胞浓度为每立方厘米10²个细胞时，消毒率为每立方厘米每小时6.0×10²个细胞。对大肠杆菌细胞进行了24小时的连续灭菌。灭菌基于电极与细胞之间由细胞内辅酶A介导的电化学反应。使用该反应器成功实现了饮用水的消毒，证明了这种反应器用于清洁高效水净化的潜力。
