水痘-带状疱疹病毒在人皮肤器官培养中的复制
Replication of varicella-zoster virus in human skin organ culture.
作者信息
Taylor Shannon L, Moffat Jennifer F
机构信息
Department of Microbiology and Immunology, SUNY Upstate Medical University, Syracuse, NY 13210, USA.
出版信息
J Virol. 2005 Sep;79(17):11501-6. doi: 10.1128/JVI.79.17.11501-11506.2005.
Abstract
Varicella-zoster virus (VZV) infection is restricted to humans, which hinders studies of its pathogenesis in rodent models of disease. To facilitate the study of VZV skin tropism, we developed an ex vivo system using human fetal skin organ culture (SOC). VZV replication was analyzed by plaque assay, transmission electron microscopy, and histology. The yield of infectious VZV from SOC increased approximately 100-fold over 6 days, virions were abundant, and lesions developed that contained VZV antigens and resembled varicella and zoster lesions. The SOC system for VZV replication has applications for testing virus mutants and antiviral drugs.
摘要
水痘带状疱疹病毒（VZV）感染仅限于人类，这阻碍了在啮齿动物疾病模型中对其发病机制的研究。为便于研究VZV的皮肤嗜性，我们开发了一种使用人胎儿皮肤器官培养（SOC）的体外系统。通过蚀斑测定、透射电子显微镜和组织学分析VZV复制情况。来自SOC的感染性VZV产量在6天内增加了约100倍，病毒粒子丰富，并且出现了含有VZV抗原且类似于水痘和带状疱疹病变的损伤。用于VZV复制的SOC系统可用于测试病毒突变体和抗病毒药物。
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