Flores-Ruiz Eric Moisés, Miranda-Novales María Guadalupe, Solórzano-Santos Fortino, Sánchez-Huerta Gustavo, Díaz-Ponce Humberto
Servicio de Infectología, Hospital de Pediatría, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Av. Cuauhtémoc 330, Col. Doctores Delegación Cuauhtémoc, México DF, CP 06780, México.
Rev Iberoam Micol. 2005 Jun;22(2):102-4. doi: 10.1016/s1130-1406(05)70017-6.
The diagnosis of candidemia by blood culture has poor sensitivity; therefore, immunossupresed patients and those with risk factors may receive empirical antifungal therapy, wich is potentially toxic. Fluorescent tests have been developed to obtain an early and more sensitive diagnosis than blood culture. The aim of this study was to compare indirect immunofluorescence vs direct calcofluor white fluorescence in buffy coat for candidemia diagnosis. Sensitivity, specificity, predictive values, of positive and negative samples were 60%, 86%, 33%, 95% and 90%, 80%, 35%, 99%, for indirect immunofluorescence and calcofluor white, respectively.
通过血培养诊断念珠菌血症的敏感性较差；因此，免疫抑制患者和有危险因素的患者可能会接受经验性抗真菌治疗，而这种治疗可能具有毒性。已开发出荧光检测方法以获得比血培养更早且更敏感的诊断。本研究的目的是比较间接免疫荧光法与在血沉棕黄层中直接使用荧光增白剂钙荧光白进行念珠菌血症诊断的效果。间接免疫荧光法和荧光增白剂钙荧光白检测阳性和阴性样本的敏感性、特异性、预测值分别为60%、86%、33%、95%和90%、80%、35%、99%。
