Kikuchi A, Kataoka K, Tsuruta T
Department of Materials Science and Technology, Faculty of Industrial Science and Technology, Science University of Tokyo, Chiba, Japan.
J Biomater Sci Polym Ed. 1992;3(3):253-60. doi: 10.1163/156856292x00169.
Adhesion and proliferation of bovine aortic endothelial cells on polystyrene derivatives having monoamine or diamine side chain was investigated focusing on the chemical structure of amino groups. Copolymers, SE8.5, is composed of polystyrene with 8.5 mol% of monoamine side chains, and SED8, which is with 8 mol% of diamine side chains, were estimated to contain almost the same amount of protonated amino groups in bulk composition at physiological pH (pH 7.4). There observed significant difference in cellular spreading of attached endothelial cells between these two types of copolymer surfaces. Spreading-% of attached cells on SED8 surfaces was approximately 1.6 times greater than that on SE8.5 6 h after seeding. This difference in cellular spreading influenced to subsequent cell growth. Cellular growth on each polymer surface was featured by parameter k, which corresponds to the 'rate constant' of cellular proliferation. While the k-value for SE8.5 decreased with decreasing seeding density as well as the case for polystyrene, SED8 maintained a high k-value even at low seeding density as 2 x 10(3) cells/cm2. These results suggest that cells may recognize the difference in the chemical structure of amine side chains of SE and SED copolymers.
以氨基的化学结构为重点，研究了牛主动脉内皮细胞在具有单胺或二胺侧链的聚苯乙烯衍生物上的黏附与增殖情况。共聚物SE8.5由含8.5 mol%单胺侧链的聚苯乙烯组成，而含8 mol%二胺侧链的SED8在生理pH值（pH 7.4）下的本体组成中估计含有几乎相同数量的质子化氨基。在这两种共聚物表面上，附着的内皮细胞的细胞铺展存在显著差异。接种6小时后，SED8表面上附着细胞的铺展率比SE8.5表面上的约高1.6倍。这种细胞铺展的差异影响了后续的细胞生长。每个聚合物表面上的细胞生长以参数k为特征，k对应于细胞增殖的“速率常数”。与聚苯乙烯的情况一样，SE8.5的k值随着接种密度的降低而降低，而SED8即使在低至2×10³个细胞/cm²的接种密度下仍保持高k值。这些结果表明，细胞可能识别出SE和SED共聚物胺侧链化学结构的差异。
