van Kooten Theo G, Spijker Hetty T, Busscher Henk J
Department of Biomedical Engineering (BME), University of Groningen, A. Deusinglaan 1, 9713 AV, Groningen, The Netherlands.
Biomaterials. 2004 May;25(10):1735-47. doi: 10.1016/j.biomaterials.2003.08.071.
Biocompatibility of biomaterials relates, amongst others, to the absence of adverse cellular reactions and modulation of cell adhesion and subsequent responses. With respect to tissue-engineering applications, most materials need to evoke cell adhesion and spreading, while potentially displaying differential cell function. Adhesion has frequently been studied in a controlled fashion, using adhesion-supporting and -inhibiting substrata. The aim of this study is to create a panel of related materials with gradually changing surface characteristics in order to sustain similar individual cell adhesion and spreading, yet different cell population behaviour. A series of polystyrene materials was created with increasing oxygen surface incorporation and, concurrently, decreasing water-contact angles. Individual cells adhered and spread on all surfaces whilst showing well-developed focal adhesions and stress fibres. Cell populations demonstrated a decreased growth on surfaces with lower wettability. The biochemical activity of cell populations was not influenced by the surface treatment, but cell proliferation on surfaces increased with increasing oxygen incorporation. Furthermore, surface coverage with assembled fibronectin matrix was higher on the substrata with higher wettability. Finally, the expression of the adhesion-related proteins cadherin-5, focal adhesion kinase and RhoA was increased on surfaces with higher wettability. Further explorations of the cell biological basis of the observed differential behaviour will give more detailed answers on the rules governing cell-material interactions.
生物材料的生物相容性尤其涉及不存在不良细胞反应以及对细胞黏附及后续反应的调节。对于组织工程应用而言，大多数材料需要引发细胞黏附和铺展，同时可能表现出不同的细胞功能。人们经常使用支持黏附和抑制黏附的基质，以可控方式研究细胞黏附。本研究的目的是创建一组具有逐渐变化表面特性的相关材料，以便维持相似的单个细胞黏附和铺展，但细胞群体行为不同。制备了一系列聚苯乙烯材料，其表面氧含量增加，同时水接触角减小。单个细胞在所有表面上均能黏附并铺展，同时显示出发育良好的黏着斑和应力纤维。细胞群体在润湿性较低的表面上生长减少。细胞群体的生化活性不受表面处理的影响，但随着氧含量的增加，细胞在表面上的增殖增加。此外，在润湿性较高的基质上，组装的纤连蛋白基质的表面覆盖率更高。最后，在润湿性较高的表面上，黏附相关蛋白钙黏蛋白-5、黏着斑激酶和RhoA的表达增加。对观察到的差异行为的细胞生物学基础进行进一步探索，将为细胞与材料相互作用的规律提供更详细的答案。
