Bhattacharjee Rama Ranjan, Das Apurba K, Haldar Debasish, Si Satyabrata, Banerjee Arindam, Mandal Tarun K
Polymer Science Unit Indian Association for the Cultivation of Science, Jadavpur, Kolkata 700 032, India.
J Nanosci Nanotechnol. 2005 Jul;5(7):1141-7. doi: 10.1166/jnn.2005.166.
A novel gold nanoparticle-tripeptide (GNP-tripeptide) conjugate was prepared by peptide in-situ redox technique at ambient temperatureusing a newly designed tripeptide. This new tripeptide was nso designed that it has a C-terminus tyrosine residue, which reduced Au+3 to Au, and the terminally located free amino group was bound to the gold nanoparticle (GNP) surface resulting in highly stable Au colloids. The average diameter of the tripeptide-stabilized GNP is 8.7 +/- 2.3 nm. Tripeptide bound gold nanoparticles formed three-dimensional assemblies in the presence of an excess of similar or disimilar tripeptides. The aggregation of GNPs results in a red shift in the surface plasmon resonance from lambda max = 527 to 556 nm. The effect of the solvent, concentration, and nature of the tripeptides on the assembly process were investigated by TEM and UV-visible spectroscopy.
采用新设计的三肽，通过肽原位氧化还原技术在室温下制备了一种新型金纳米颗粒 - 三肽（GNP - 三肽）共轭物。这种新设计的三肽具有一个C末端酪氨酸残基，该残基将Au +3还原为Au，并且末端的游离氨基与金纳米颗粒（GNP）表面结合，从而形成高度稳定的金胶体。三肽稳定的GNP的平均直径为8.7 +/- 2.3 nm。在存在过量的相似或不同三肽的情况下，三肽结合的金纳米颗粒形成三维聚集体。GNP的聚集导致表面等离子体共振从λmax = 527 nm红移至556 nm。通过透射电子显微镜（TEM）和紫外 - 可见光谱研究了溶剂、三肽浓度和性质对组装过程的影响。
